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AB103159

Anti-Granzyme B antibody [CLB-GB11]

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(6 Publications)

Mouse Monoclonal Granzyme B antibody. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human GZMB.

View Alternative Names

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

2 Images
Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [CLB-GB11] (AB103159)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [CLB-GB11] (AB103159)

Separation of Granzyme B positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using ab103159 at 3 μg/ml.

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [CLB-GB11] (AB103159)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [CLB-GB11] (AB103159)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using ab103159 at 3 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CLB-GB11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Native Full Length Protein corresponding to Human GZMB.

P10144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-4 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from cell culture supernatant.Purity > 95% (by SDS-PAGE)
Storage buffer
pH: 8 Preservative: 0.097% Sodium azide Constituents: Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
See full target information GZMB
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Endocrine connections 13: PubMed39235859

2024

The role of PANDER and its interplay with IL-6 in the regulation of GLP-1 secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zeting Li,Ling Pei,Huangmeng Xiao,Nan Chen,Fenghua Lai,Shufang Yue,Changliu Xu,Yanbing Li,Haipeng Xiao,Xiaopei Cao

Nature 629:426-434 PubMed38658764

2024

PGE inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Morotti,Alizee J Grimm,Helen Carrasco Hope,Marion Arnaud,Mathieu Desbuisson,Nicolas Rayroux,David Barras,Maria Masid,Baptiste Murgues,Bovannak S Chap,Marco Ongaro,Ioanna A Rota,Catherine Ronet,Aspram Minasyan,Johanna Chiffelle,Sebastian B Lacher,Sara Bobisse,Clément Murgues,Eleonora Ghisoni,Khaoula Ouchen,Ribal Bou Mjahed,Fabrizio Benedetti,Naoill Abdellaoui,Riccardo Turrini,Philippe O Gannon,Khalil Zaman,Patrice Mathevet,Loic Lelievre,Isaac Crespo,Marcus Conrad,Gregory Verdeil,Lana E Kandalaft,Julien Dagher,Jesus Corria-Osorio,Marie-Agnes Doucey,Ping-Chih Ho,Alexandre Harari,Nicola Vannini,Jan P Böttcher,Denarda Dangaj Laniti,George Coukos

eLife 12: PubMed36688525

2023

Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Gerdien Mijnheer,Nila Hendrika Servaas,Jing Yao Leong,Arjan Boltjes,Eric Spierings,Phyllis Chen,Liyun Lai,Alessandra Petrelli,Sebastiaan Vastert,Rob J de Boer,Salvatore Albani,Aridaman Pandit,Femke van Wijk

Cell 185:916-938.e58 PubMed35216673

2022

A blood atlas of COVID-19 defines hallmarks of disease severity and specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Cell 184:3394-3409.e20 PubMed34077752

2021

Microbial exposure during early human development primes fetal immune cells.

Applications

Unspecified application

Species

Unspecified reactive species

Archita Mishra,Ghee Chuan Lai,Leong Jing Yao,Thet Tun Aung,Noam Shental,Aviva Rotter-Maskowitz,Edwin Shepherdson,Gurmit Singh Naranjan Singh,Rhea Pai,Adhika Shanti,Regina Men Men Wong,Andrea Lee,Costerwell Khyriem,Charles Antoine Dutertre,Svetoslav Chakarov,K G Srinivasan,Nurhidaya Binte Shadan,Xiao-Meng Zhang,Shabnam Khalilnezhad,Fabien Cottier,Alrina Shin Min Tan,Gillian Low,Phyllis Chen,Yiping Fan,Pei Xiang Hor,Avery Khoo May Lee,Mahesh Choolani,David Vermijlen,Ankur Sharma,Garold Fuks,Ravid Straussman,Norman Pavelka,Benoit Malleret,Naomi McGovern,Salvatore Albani,Jerry Kok Yen Chan,Florent Ginhoux

Cell reports. Medicine 2:100278 PubMed34095880

2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Angeline Rouers,Melissa Hui Yen Chng,Bernett Lee,Menaka P Rajapakse,Kaval Kaur,Ying Xiu Toh,Durgalakshmi Sathiakumar,Thomas Loy,Tun-Linn Thein,Vanessa W X Lim,Amit Singhal,Tsin Wen Yeo,Yee-Sin Leo,Kalpit A Vora,Danilo Casimiro,Bing Lim,Lisa Tucker-Kellogg,Laura Rivino,Evan W Newell,Katja Fink
View all publications
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