Anti-Granzyme B [EPR20129-217] antibody (ab219803) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect Granzyme B in Western Blot, IHC-P, mIHC. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38390.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img53865.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803)")
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401811.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803)")
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401808.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803)")
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401812.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803)")
Publications
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- Cell reports methods 1:2021Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik A Burlingame,Jennifer Eng,Guillaume Thibault,Koei Chin,Joe W Gray,Young Hwan ChangPubMed 34485971
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | WB | Flow Cyt | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
3 products for Alternative Version
Target data
Function
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:1985927, PubMed:3262682, PubMed:3263427). It cleaves after Asp (PubMed:1985927, PubMed:8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:31953257, PubMed:32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed:9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
Alternative names
CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP
Recommended products
Anti-Granzyme B [EPR20129-217] antibody (ab219803) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect Granzyme B in Western Blot, IHC-P, mIHC. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20129-217
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Multiplex IHC (mIHC) in Human samples.
What is the molecular weight of Granzyme B?
Anti-Granzyme B [EPR20129-217] - BSA and Azide free (ab219803) specifically detects a band for Granzyme B (UniProt: P10144) at a molecular weight of 28kDa.
Reviewed by scientists
Anti-Granzyme B [EPR20129-217] - BSA and Azide free (ab219803) has over 5 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [EPR20129-217] also available for your convenience:
Anti-Granzyme B antibody [EPR20129-217] ab208586, Carrier free - ab219803, PE - PE Anti-Granzyme B antibody [EPR20129-217] ab225471, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217] ab225472, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Granzyme B antibody [EPR20129-217] ab225473, Carrier free - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (Detector) ab245038, Carrier free - ab307493
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
- Biological function summary
Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.
- Pathways
Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.
- Associated diseases and disorders
Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38390.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
This IHC data was generated using the same anti-Granzyme B antibody clone [EPR20129-217] in a different buffer formulation (cat# Anti-Granzyme B antibody [EPR20129-217] ab208586).
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling Granzyme B with Anti-Granzyme B antibody [EPR20129-217] ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on neutrophils and stroma cells of human cervix cancer is observed [PMID: 14512315]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img53865.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Granzyme B with Anti-Granzyme B antibody [EPR20129-217] ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on some stromal cells of human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granzyme B antibody [EPR20129-217] ab208586).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401811.jpg "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data is courtesy of ImmunoAtlas and it can be found here.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granzyme B antibody [EPR20129-217] ab208586).
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401808.jpg "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data is courtesy of ImmunoAtlas and it can be found here.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granzyme B antibody [EPR20129-217] ab208586).![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401812.jpg "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granzyme B antibody [EPR20129-217] ab208586).
This data is courtesy of ImmunoAtlas and it can be found here.
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401809.jpg "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granzyme B antibody [EPR20129-217] ab208586).
This data is courtesy of ImmunoAtlas and it can be found here.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423078.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Tissue Microarrays stained for "Anti-Granzyme B antibody [EPR20129-217]" using "Anti-Granzyme B antibody [EPR20129-217] ab208586"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-Granzyme B antibody [EPR20129-217] ab208586 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401810.jpg "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granzyme B antibody [EPR20129-217] ab208586).
This data is courtesy of ImmunoAtlas and it can be found here.
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401809.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423078.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
![Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803), expandable thumbnail](https://content.abcam.com/products/images/granzyme-b-antibody-epr20129-217-bsa-and-azide-free-ab219803--multiplex-immunohistochemistry-img401810.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803)")
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