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AB255599

Anti-Granzyme B antibody [EPR22645-244]

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(3 Publications)

Rabbit Recombinant Monoclonal Granzyme B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

Ctla-1, Ctla1, Gzmb, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, CCP1

2 Images
Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR22645-244] (AB255599)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR22645-244] (AB255599)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte (treated with 2.5ug/ml Concanavalin A for 72h) cells labelling Granzyme B with ab255599 at 1/800 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD8 alpha conjugated to Alexa Fluor® 647.Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab255599 (Right).

Western blot - Anti-Granzyme B antibody [EPR22645-244] (AB255599)
  • WB

Unknown

Western blot - Anti-Granzyme B antibody [EPR22645-244] (AB255599)

The expression of Granzyme B is elevated in stimulated splenocytes. Granzyme B forms a complex with its protease inhibitor. The molecular weight observed is consistent with what has been described in the literature (PMID : 8663264, PMID : 17116752, PMID : 21919028, PMID : 9774654).

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Granzyme B antibody [EPR22645-244] (ab255599) at 1/1000 dilution

Lane 1:

Untreated mouse splenocyte lysate at 20 µg

Lane 2:

Mouse splenocyte (treated with 2.5μg/ml Concanavalin for 72 hours ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 27 kDa,65 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22645-244

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/800", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 35705808). It cleaves after Asp (PubMed : 35705808). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (By similarity). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution (By similarity). Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 35705808). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed : 35705808).
See full target information Gzmb
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112988 PubMed41054545

2025

Multiregional single-cell transcriptomics reveals an association between partial EMT and immunosuppressive states in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghoon Kim,Hyun Jung Kee,Dahee Kim,Jinho Jang,Hyoung-Oh Jeong,Nam Suk Sim,Mischa Selig,Jana Ihlow,Livius Penter,Taejoo Hwang,David Whee-Young Choi,Kyoung Jun Lee,Jaewoong Lee,Young Min Park,Semin Lee,Yoon Woo Koh

Histology and histopathology 40:1061-1071 PubMed39410869

2024

DNMT1 silencing induces KIR2DL1/2/3 expression via methylation to alleviate graft--host disease after allogeneic hematopoietic stem cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhang,Shuling Yu,Miao Zhou,Xiao Yan,Huiling Zhu,Lixia Sheng,Yi Zhang,Shujun Yang,Guifang Ouyang

Cell genomics 4:100659 PubMed39317187

2024

CXCR4 orchestrates the TOX-programmed exhausted phenotype of CD8 T cells via JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Canhui Cao,Miaochun Xu,Ye Wei,Ting Peng,Shitong Lin,Xiaojie Liu,Yashi Xu,Tian Chu,Shiyi Liu,Ping Wu,Bai Hu,Wencheng Ding,Li Li,Ding Ma,Peng Wu
View all publications
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