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AB237847

Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free

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(4 Publications)

Mouse Monoclonal Granzyme B antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GZMB aa 50-200.

View Alternative Names

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Granzyme B using ab237847 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Granzyme B using ab237847 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)
  • Protein Array

Supplier Data

Protein Array - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)

Analysis of Protein Array containing >19,000 full-length human proteins using ab237847.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GZMB/2403

Isotype

IgG2b

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GZMB aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P10144

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
See full target information GZMB

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40563509

2025

Positive Effects of (+)-Epicatechin on Traumatic Spinal Cord Injury Recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Gonzalez-Ruiz,Rodrigo Mondragón-Lozano,Hermelinda Salgado-Ceballos,Francisco Villarreal,Yuridia Martínez-Meza,Eduardo Meaney,Nayelli Nájera,Guillermo Ceballos

PLoS pathogens 20:e1012768 PubMed39652618

2024

Fatal Lassa fever in cynomolgus monkeys is associated with systemic viral dissemination and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jimmy Hortion,Emeline Perthame,Blaise Lafoux,Laura Soyer,Stéphanie Reynard,Alexandra Journeaux,Clara Germain,Hélène Lopez-Maestre,Natalia Pietrosemoli,Nicolas Baillet,Séverine Croze,Catherine Rey,Catherine Legras-Lachuer,Sylvain Baize

International journal of oral science 16:29 PubMed38622125

2024

Single cell analysis unveils B cell-dominated immune subtypes in HNSCC for enhanced prognostic and therapeutic stratification.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Caihua Zhang,Ruoxing Zhou,Maosheng Cheng,Rongsong Ling,Gan Xiong,Jieyi Ma,Yan Zhu,Shuang Chen,Jie Chen,Demeng Chen,Liang Peng

Science translational medicine 13: PubMed33568521

2021

Targeted immunotherapy for HER2-low breast cancer with 17p loss.

Applications

Unspecified application

Species

Unspecified reactive species

Yujing Li,Yifan Sun,Michael Kulke,Torsten Hechler,Kevin Van der Jeught,Tianhan Dong,Bin He,Kathy D Miller,Milan Radovich,Bryan P Schneider,Andreas Pahl,Xinna Zhang,Xiongbin Lu
View all publications

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