Anti-Granzyme K antibody [EPR24601-164]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. (PMID : 22207739). The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human cervical carcinoma. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human diffuse large B-cell lymphoma. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human NKT lymphoma tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human NKT lymphoma. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human pancreas. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling Granzyme K with 282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes in human endometrium. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 10407175, 18559365).

Negative control : heart (PMID : 10407175).

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Granzyme K antibody [EPR24601-164] (ab282703) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 40 µg

Lane 2:

Human tonsil tissue lysate at 40 µg

Lane 3:

Human lymphoma tissue lysate at 40 µg

Lane 4:

Human heart tissue lysate at 40 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 22 kDa,27 kDa

false