Rabbit Recombinant Monoclonal Granzyme K antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--western-blot-img173943.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164361.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181598.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170125.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181051.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (AB282714)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Alternative names
TRYP2, GZMK, Granzyme K, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2
Recommended products
Rabbit Recombinant Monoclonal Granzyme K antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24601-164
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab282714 is the carrier-free version of Anti-Granzyme K antibody [EPR24601-164] ab282703.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Granzyme K also known as protein K or 24c3 is a serine protease with a mass of approximately 27 kilodaltons. It belongs to the granzyme family of proteins which are mainly found within the cytotoxic granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). These immune cells store granzyme K in their granules which they release to induce apoptosis in target cells. Researchers have identified granzyme K expression in various tissues and immune cells highlighting its widespread role in modulating immune responses.
- Biological function summary
Granzyme K serves as a potent mediator in the immune system by inducing apoptosis in target cells. It does not act alone but rather in concert with other granzymes and proteins to effectively execute cell death. Granzyme K can degrade cellular proteins once introduced into a target cell's cytoplasm leading to apoptosis. The protease functions independently and does not require a complex for its activity contributing to the immune system's ability to eliminate infected or transformed cells.
- Pathways
The granzyme K protein exists within essential immune regulatory pathways such as the cytotoxic T lymphocyte-induced apoptosis pathway and the natural killer cell-mediated cytotoxicity pathway. Granzyme K acts alongside granzymes A and B to promote programmed cell death and finely tune host immune responses. While granzymes A and B have more defined roles granzyme K complements their activity offering redundancy and ensuring efficient elimination of harmful target cells.
- Associated diseases and disorders
Granzyme K shows significant connections to inflammatory conditions and autoimmune diseases such as rheumatoid arthritis. Elevated granzyme K levels in these diseases may result from dysregulated immune responses leading to excessive tissue damage. In autoimmune disorders granzyme K works in combination with proteins like perforin which facilitates entry of granzymes into the cell to stimulate apoptosis. Understanding granzyme K's role in these conditions may help develop therapeutic approaches to modulate immune activity and minimize tissue damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--western-blot-img173943.jpg "Western blot - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Western blot - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 10407175, 18559365).
Negative control: heart (PMID: 10407175).
Exposure time: 15 seconds
All lanes: Western blot - Anti-Granzyme K antibody [EPR24601-164] (Anti-Granzyme K antibody [EPR24601-164] ab282703) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 40 µg
Lane 2: Human tonsil tissue lysate at 40 µg
Lane 3: Human lymphoma tissue lysate at 40 µg
Lane 4: Human heart tissue lysate at 40 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 22 kDa, 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164361.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Granzyme K with Anti-Granzyme K antibody [EPR24601-164] ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. (PMID: 22207739). The section was incubated with Anti-Granzyme K antibody [EPR24601-164] ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181598.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labelling Granzyme K with Anti-Granzyme K antibody [EPR24601-164] ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human diffuse large B-cell lymphoma. The section was incubated with Anti-Granzyme K antibody [EPR24601-164] ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170125.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling Granzyme K with 282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes in human endometrium. The section was incubated with Anti-Granzyme K antibody [EPR24601-164] ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181051.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human NKT lymphoma tissue labelling Granzyme K with Anti-Granzyme K antibody [EPR24601-164] ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human NKT lymphoma. The section was incubated with Anti-Granzyme K antibody [EPR24601-164] ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167403.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling Granzyme K with Anti-Granzyme K antibody [EPR24601-164] ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human cervical carcinoma. The section was incubated with Anti-Granzyme K antibody [EPR24601-164] ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175465.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)
This data was developed using Anti-Granzyme K antibody [EPR24601-164] ab282703, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling Granzyme K with Anti-Granzyme K antibody [EPR24601-164] ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human pancreas. The section was incubated with Anti-Granzyme K antibody [EPR24601-164] ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167403.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714), expandable thumbnail](https://content.abcam.com/products/images/granzyme-k-antibody-epr24601-164-bsa-and-azide-free-ab282714--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175465.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] - BSA and Azide free (ab282714)")
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