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AB224613

Anti-GRAP2 antibody

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(1 Publication)

Rabbit Polyclonal GRAP2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRAP2 aa 100-300.

View Alternative Names

GADS, GRB2L, GRID, GRAP2, GRB2-related adapter protein 2, Adapter protein GRID, GRB-2-like protein, GRBLG, GRBX, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, P38, Protein GADS, SH3-SH2-SH3 adapter Mona, Grf-40

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody (AB224613)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody (AB224613)

Paraffin-embedded human lymph node tissue stained for GRAP2 using ab224613 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GRAP2 antibody (AB224613)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRAP2 antibody (AB224613)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRAP2 (green) using ab224613 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-GRAP2 antibody (AB224613)
  • WB

Supplier Data

Western blot - Anti-GRAP2 antibody (AB224613)

All lanes:

Western blot - Anti-GRAP2 antibody (ab224613) at 1/500 dilution

All lanes:

Rat liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 37 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRAP2 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

O75791

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GRAP2 also known as Gads plays an important role in intracellular signaling mechanisms. It is an adaptor protein with a mass of about 37 kDa. GRAP2 predominantly expresses in hematopoietic cells where it facilitates specific downstream signaling pathways. The protein assists in linking cell surface receptors to downstream signaling molecules enabling effective transmission of cellular signals.
Biological function summary

GRAP2 participates in the immune response by acting as a mediator that aids the assembly of signaling complexes. It often interacts with other proteins such as SLP-76 which is involved in specific immune cell activation. GRAP2 forms part of larger signaling complexes which are critical for the proper development and function of immune cells. This role highlights its importance in maintaining efficient immune surveillance and response.

Pathways

GRAP2 significantly contributes to the T-cell receptor (TCR) signaling pathway. It plays an integral part by interacting with proteins like LAT and ZAP-70 providing a scaffold for the further propagation of activation signals. Additionally in the Ras signaling pathway GRAP2 associates with other adaptor proteins coordinating downstream signaling needed for T cell activation and function.

GRAP2 has associations with immune system Pact conditions notably autoimmune diseases such as systemic lupus erythematosus (SLE). Its interactions with proteins like LAT and SLP-76 are important in these dysregulation of immune responses seen in these disorders. Any alteration in GRAP2 function or expression pathways could therefore potentially contribute to pathological immune activation reflecting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
See full target information GRAP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 69:1349-1358 PubMed36305691

2022

PHF21A expression as a biomarker of hepatocellular carcinoma progression and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Qing Huang,Li-Chen Ji,Qiang-An Jing,Yi-Chen He,Ying-Yu Ma,Xiang-Min Tong
View all publications
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Product promise

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