AB171940

Anti-GRASP1 antibody [EPR11703]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GRASP1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1167, GRIPAP1, GRIP1-associated protein 1, GRASP-1

2 Images

Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] (AB171940)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] (AB171940)

Intracellular flow cytometric analysis of permeabilized HeLa labeling GRASP1 with ab171940 at 1/10 (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-GRASP1 antibody [EPR11703] (AB171940)

All lanes:

Western blot - Anti-GRASP1 antibody [EPR11703] (ab171940) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

HepG2 cell line lysate at 10 µg

Lane 3:

HeLa cell line lysate at 10 µg

Predicted band size: 96 kDa

true

Carrier free

Anti-GRASP1 antibody [EPR11703] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11703

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRASP1 also known as GRIP1-associated protein 1 is a scaffold protein involved in trafficking in neurons. It is part of the GRIP (Glutamate Receptor Interacting Protein) family and has a molecular weight of about 90 kDa. GRASP1 is expressed in the brain especially in areas such as the hippocampus and cortex. Its function includes facilitating the transport and stability of proteins at synaptic sites playing a role in synaptic plasticity and signaling.

Biological function summary

GRASP1 interacts with other proteins to modulate the assembly and function of the AMPA receptor complex at synapses. It is important for proper neuronal signaling and communication as it organizes the cellular machinery required for synaptic strength. GRASP1 works as part of the AMPA receptor complex affecting neurotransmitter regulation and synaptic transmission efficiency. This protein's role in regulating synaptic receptor composition influences learning and memory processes in the brain.

Pathways

GRASP1 integrates into the glutamatergic signaling pathway impacting neural communication and plasticity. It is associated with regulating the trafficking pathways of AMPA receptors important for excitatory neurotransmission. GRASP1 interacts with proteins like GRIP1 and PICK1 which help in maintaining the receptor composition at synaptic junctions. These interactions sustain the dynamic modulation of synaptic strength an essential component of the nervous system's flexibility.

GRASP1 connects to neurodegenerative disorders like Alzheimer's disease. Changes in its function can contribute to synaptic dysfunction observed in such conditions. GRASP1 has been linked to proteins involved in neurotransmitter regulation and its dysregulation may influence the pathology of synaptic failure. In addition altered expression or function of GRASP1 has been associated with certain cognitive disorders further implicating its significance in maintaining synaptic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).. GRASP-1 C-terminal chain. Functions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity.

See full target information GRIPAP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 17:1173-1181 PubMed29115485

2017

Effect of willed movement training on neurorehabilitation after focal cerebral ischemia and on the neural plasticity-associated signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Wen Zhou,Qi-Dong Yang,Qing-Ping Tang,Jie Yang,Rong-Jing Guo,Wen Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRASP1 antibody [EPR11703]

Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] (AB171940)

Western blot - Anti-GRASP1 antibody [EPR11703] (AB171940)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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