Rabbit Recombinant Monoclonal GRASP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660), expandable thumbnail](https://content.abcam.com/products/images/grasp1-antibody-epr11703-bsa-and-azide-free-ab249660--flow-cytometry-intracellular-img27175.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660)")
![Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660), expandable thumbnail](https://content.abcam.com/products/images/grasp1-antibody-epr11703-bsa-and-azide-free-ab249660--western-blot-img70256.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC | WB | ICC/IF | Flow Cyt (Intra) | IHC-FrFl | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Predicted | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-FrFl
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity). GRASP-1 C-terminal chain. Functions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity.
Alternative names
KIAA1167, GRIPAP1, GRIP1-associated protein 1, GRASP-1
Recommended products
Rabbit Recombinant Monoclonal GRASP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11703
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249660 is the carrier-free version of Anti-GRASP1 antibody [EPR11703] ab171940.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRASP1 also known as GRIP1-associated protein 1 is a scaffold protein involved in trafficking in neurons. It is part of the GRIP (Glutamate Receptor Interacting Protein) family and has a molecular weight of about 90 kDa. GRASP1 is expressed in the brain especially in areas such as the hippocampus and cortex. Its function includes facilitating the transport and stability of proteins at synaptic sites playing a role in synaptic plasticity and signaling.
- Biological function summary
GRASP1 interacts with other proteins to modulate the assembly and function of the AMPA receptor complex at synapses. It is important for proper neuronal signaling and communication as it organizes the cellular machinery required for synaptic strength. GRASP1 works as part of the AMPA receptor complex affecting neurotransmitter regulation and synaptic transmission efficiency. This protein's role in regulating synaptic receptor composition influences learning and memory processes in the brain.
- Pathways
GRASP1 integrates into the glutamatergic signaling pathway impacting neural communication and plasticity. It is associated with regulating the trafficking pathways of AMPA receptors important for excitatory neurotransmission. GRASP1 interacts with proteins like GRIP1 and PICK1 which help in maintaining the receptor composition at synaptic junctions. These interactions sustain the dynamic modulation of synaptic strength an essential component of the nervous system's flexibility.
- Associated diseases and disorders
GRASP1 connects to neurodegenerative disorders like Alzheimer's disease. Changes in its function can contribute to synaptic dysfunction observed in such conditions. GRASP1 has been linked to proteins involved in neurotransmitter regulation and its dysregulation may influence the pathology of synaptic failure. In addition altered expression or function of GRASP1 has been associated with certain cognitive disorders further implicating its significance in maintaining synaptic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (ab249660), expandable thumbnail](https://content.abcam.com/products/images/grasp1-antibody-epr11703-bsa-and-azide-free-ab249660--flow-cytometry-intracellular-img27175.jpg "Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (ab249660)")
Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (ab249660)
This data was developed using Anti-GRASP1 antibody [EPR11703] ab171940, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized Hela labeling GRASP1 with Anti-GRASP1 antibody [EPR11703] ab171940 at 1/10 (red) or a rabbit IgG (negative) (green).
![Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (ab249660), expandable thumbnail](https://content.abcam.com/products/images/grasp1-antibody-epr11703-bsa-and-azide-free-ab249660--western-blot-img70256.jpg "Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (ab249660)")
Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (ab249660)
This data was developed using Anti-GRASP1 antibody [EPR11703] ab171940, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GRASP1 antibody [EPR11703] (Anti-GRASP1 antibody [EPR11703] ab171940) at 1/10000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: HepG2 cell line lysate at 10 µg
Lane 3: HeLa cell line lysate at 10 µg
Developed using the ECL technique.
Predicted band size: 96 kDa
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