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AB249660

Anti-GRASP1 antibody [EPR11703] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GRASP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1167, GRIPAP1, GRIP1-associated protein 1, GRASP-1

2 Images
Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660)

This data was developed using ab171940, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Hela labeling GRASP1 with ab171940 at 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660)
  • WB

Supplier Data

Western blot - Anti-GRASP1 antibody [EPR11703] - BSA and Azide free (AB249660)

This data was developed using ab171940, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRASP1 antibody [EPR11703] (<a href='/en-us/products/primary-antibodies/grasp1-antibody-epr11703-ab171940'>ab171940</a>) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

HepG2 cell line lysate at 10 µg

Lane 3:

HeLa cell line lysate at 10 µg

Predicted band size: 96 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11703

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249660 is the carrier-free version of ab171940.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRASP1 also known as GRIP1-associated protein 1 is a scaffold protein involved in trafficking in neurons. It is part of the GRIP (Glutamate Receptor Interacting Protein) family and has a molecular weight of about 90 kDa. GRASP1 is expressed in the brain especially in areas such as the hippocampus and cortex. Its function includes facilitating the transport and stability of proteins at synaptic sites playing a role in synaptic plasticity and signaling.
Biological function summary

GRASP1 interacts with other proteins to modulate the assembly and function of the AMPA receptor complex at synapses. It is important for proper neuronal signaling and communication as it organizes the cellular machinery required for synaptic strength. GRASP1 works as part of the AMPA receptor complex affecting neurotransmitter regulation and synaptic transmission efficiency. This protein's role in regulating synaptic receptor composition influences learning and memory processes in the brain.

Pathways

GRASP1 integrates into the glutamatergic signaling pathway impacting neural communication and plasticity. It is associated with regulating the trafficking pathways of AMPA receptors important for excitatory neurotransmission. GRASP1 interacts with proteins like GRIP1 and PICK1 which help in maintaining the receptor composition at synaptic junctions. These interactions sustain the dynamic modulation of synaptic strength an essential component of the nervous system's flexibility.

GRASP1 connects to neurodegenerative disorders like Alzheimer's disease. Changes in its function can contribute to synaptic dysfunction observed in such conditions. GRASP1 has been linked to proteins involved in neurotransmitter regulation and its dysregulation may influence the pathology of synaptic failure. In addition altered expression or function of GRASP1 has been associated with certain cognitive disorders further implicating its significance in maintaining synaptic health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).. GRASP-1 C-terminal chain. Functions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity.
See full target information GRIPAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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