Rabbit Recombinant Monoclonal GRASP65 antibody. C-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunocytochemistry-immunofluorescence-img20745.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834)")
![Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--western-blot-img57322.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120492.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81673.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834)")
![Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunoprecipitation-img72588.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834)")
Publications
Filter by
- Cell communication and signaling : CCS 21:1562023The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShao-Chih Chiu,Xin-Ting Yang,Tong-You Wade Wei,Yu-Ting Amber Liao,Jo-Mei Maureen Chen,Yi-Chun Kuo,Chun-Chih Jared Liu,Chiao-Yun Cheng,Yu-Ting Jenny Huang,Yun-Ru Jaoying Huang,He-Lian Joe Wu,Chang-Xin Wan,Jia-Rung Tsai,Chang-Tze Ricky YuPubMed 37370099
- Journal of cellular physiology 237:4517-45302022The JMJD6/HURP axis promotes cell migration via NF-κB-dependent centrosome repositioning and Cdc42-mediated Golgi repositioning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Ru Jaoying Huang,Shao-Chih Chiu,Jeng-Sen Tseng,Jo-Mei Maureen Chen,Tong-You Wade Wei,Chen-Yu Chu,Hsu-Ting Eric Kao,Chieh-Yun Oprah Yang,Yong-Chun Erin Shih,Tsung-Ying Yang,Kun-Yuan Chiu,Chieh-Lin Jerry Teng,Chang-Tze Ricky YuPubMed 36250981
- Neuroscience bulletin 38:249-2622021RhoGEF Trio Regulates Radial Migration of Projection Neurons via Its Distinct Domains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengwen Wei,Mengwen Sun,Xiaoxuan Sun,Hu Meng,Qiongwei Li,Kai Gao,Weihua Yue,Lifang Wang,Dai Zhang,Jun LiPubMed 34914033
- Communications biology 4:13702021Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek LeePubMed 34876695
- Journal of cellular physiology 237:1033-10432021The PRMT5/HURP axis retards Golgi repositioning by stabilizing acetyl-tubulin and Golgi apparatus during cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShao-Chih Chiu,Yun-Ru Jaoying Huang,Tong-You Wade Wei,Jo-Mei Maureen Chen,Yi-Chun Kuo,Yu-Ting Jenny Huang,Yu-Ting Amber Liao,Chang-Tze Ricky YuPubMed 34541678
- International journal of molecular sciences 22:2021Regulation of the Golgi Apparatus by p38 and JNK Kinases during Cellular Stress Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCathrine Nordgaard,Maxim A X Tollenaere,Ana Martinez Del Val,Dorte B Bekker-Jensen,Melanie Blasius,Jesper V Olsen,Simon Bekker-JensenPubMed 34502507
- Biochemical and biophysical research communications 527:406-4102020Markers of malignant prostate cancer cells: Golgi localization of α-mannosidase 1A at GM130-GRASP65 site and appearance of high mannose N-glycans on cell surface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPi-Wan Cheng,Samuel Davidson,Ganapati BhatPubMed 32331836
- Nature methods 17:225-2312020Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongdeng Zhang et. al.PubMed 31907447
- Cells 8:2019Post-ER Stress Biogenesis of Golgi Is Governed by Giantin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCole P Frisbie et. al.PubMed 31847122
- Nature communications 10:43072019Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Chia Chang et. al.PubMed 31541088
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
Target data
Function
Key structural protein of the Golgi apparatus (PubMed:33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed:33301566). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed:33301566). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (By similarity). Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (PubMed:16489344). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed:21884936).
Alternative names
GOLPH5, GRASP65, GORASP1, Golgi reassembly-stacking protein 1, Golgi peripheral membrane protein p65, Golgi phosphoprotein 5, Golgi reassembly-stacking protein of 65 kDa
Recommended products
Rabbit Recombinant Monoclonal GRASP65 antibody. C-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12439
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRASP65 also known as Golgi reassembly stacking protein 65 is an important Golgi apparatus-associated protein. It weighs approximately 74 kDa and is expressed predominantly in mammalian cells. GRASP65 localizes to the cis-Golgi cisternae performing functions necessary for preserving the Golgi structure and facilitating protein trafficking. This protein plays a role in maintaining the Golgi ribbon during the cell cycle which is essential for proper cellular operation.
- Biological function summary
GRASP65 participates in key processes related to Golgi stack organization and structure. It forms a complex with other Golgi proteins acting as an important scaffold protein during membrane tethering and linking adjacent Golgi membranes. GRASP65 influences Golgi dynamics by regulating Golgi stacking and membrane fusion events which are essential processes for the normal functioning of the secretory pathway.
- Pathways
GRASP65 integrates into the mitotic Golgi fragmentation pathway and also influences the ER-to-Golgi transport pathway. It interacts with proteins such as GM130 and forms a bridge between vesicles and Golgi membranes influencing vesicle tethering and cisternal formation. In these roles GRASP65 helps coordinate Golgi disassembly and reassembly processes that are important during cell division to ensure proper Golgi inheritance among daughter cells.
- Associated diseases and disorders
GRASP65 displays relationships with certain neurodegenerative and cancerous conditions. Dysfunctional GRASP65 activity associates with Alzheimer's disease-related pathologies potentially linked through proteins such as tau which aggregates during disease progression. It also connects to certain cancers by misregulating Golgi structure suggesting a role in improper cell cycle control. Understanding GRASP65's function and regulation could offer insights into therapeutic strategies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunocytochemistry-immunofluorescence-img20745.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
Immunofluorescent analysis of HepG2 cells, labeling GRASP65 with ab174834 at 1/100 dilution (red). DAPI nuclear staining (blue).
![Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--western-blot-img57322.jpg "Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
All lanes: Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: MCF7 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 46 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120492.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GRASP65 with ab174834 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81673.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, labeling GRASP65 with ab174834 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--immunoprecipitation-img72588.jpg "Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling GRASP65, immunoprecipitated using ab174834 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
Developed using the ECL technique.
Predicted band size: 46 kDa
![Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--flow-cytometry-intracellular-img27342.jpg "Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling GRASP65with ab174834 at 1/10 dilution (red), or negative control rabbit IgG (green).
![Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834), expandable thumbnail](https://content.abcam.com/products/images/grasp65-antibody-epr12439-c-terminal-ab174834--flow-cytometry-intracellular-img27342.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)")
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