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AB125583

Anti-GRB10 antibody

3

(4 Reviews)

|

(2 Publications)

Rabbit Polyclonal GRB10 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GRB10.

View Alternative Names

GRBIR, KIAA0207, GRB10, Growth factor receptor-bound protein 10, GRB10 adapter protein, Insulin receptor-binding protein Grb-IR

1 Images
Western blot - Anti-GRB10 antibody (AB125583)
  • WB

Unknown

Western blot - Anti-GRB10 antibody (AB125583)

Secondary antibody - anti-rabbit HRP (ab6721)

Lane 1:

Western blot - Anti-GRB10 antibody (ab125583) at 1 µg/mL

Lane 2:

Western blot - Anti-GRB10 antibody (ab125583) at 2 µg/mL

All lanes:

SK-N-SH cell lysate at 15 µg

Predicted band size: 67 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GRB10. The exact immunogen used to generate this antibody is proprietary information.

Q13322

Specificity

At least three isoforms of GRB10 are known to exist; ab125583 will detect all three isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Growth Factor Receptor-Bound Protein 10 often referred to as GRB10 is an adaptor protein with a mass of approximately 66 kDa. This protein interacts with various receptor tyrosine kinases and other intracellular signaling molecules. GRB10 is expressed in many tissues but it shows higher expression in the brain muscle and liver. It plays a significant role as a modulator in cellular processes that involve signal transduction.
Biological function summary

GRB10 interacts with receptor tyrosine kinases such as the insulin receptor and engages in regulating the insulin signaling pathway. It acts as part of a complex that influences cell growth and metabolism. GRB10 binds Via its SH2 domain to activated receptors such as IGF-1R altering downstream signaling and having an impact on growth and metabolic processes.

Pathways

GRB10 is integral to the PI3K-AKT signaling pathway and the mTOR pathway. It negatively regulates these pathways by binding to receptors and altering downstream effects. GRB10 interacts with proteins such as IRS1 and mTORC1 affecting how cells respond to insulin and growth factors. Through these interactions it contributes to cellular metabolism and growth regulation.

Studies show that GRB10 has connections to conditions like cancer and type 2 diabetes. Alterations in GRB10's function or expression can lead to abnormal cellular signaling contributing to oncogenesis due to disrupted growth signals. In type 2 diabetes GRB10 affects insulin signaling pathways which is related to insulin resistance. These disorders show interconnection with proteins like IRS1 where GRB10 modulates pathways influencing disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms : interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
See full target information GRB10
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:3522492 PubMed35571247

2022

circHtra1/miR-3960/GRB10 Axis Promotes Neuronal Loss and Immune Deficiency in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zheng,Liang Shu,Dabin Ren,Zhucai Kuang,Yisong Zhang,Jian Wan

Scientific reports 8:1366 PubMed29358694

2018

Maternal whole blood cell miRNA-340 is elevated in gestational diabetes and inversely regulated by glucose and insulin.

Applications

Unspecified application

Species

Human

Laura Stirm,Peter Huypens,Steffen Sass,Richa Batra,Louise Fritsche,Sara Brucker,Harald Abele,Anita M Hennige,Fabian Theis,Johannes Beckers,Martin Hrabě de Angelis,Andreas Fritsche,Hans-Ulrich Häring,Harald Staiger
View all publications
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