Rabbit Polyclonal GRB10 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRB10 aa 350 to C-terminus.
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Publications
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- Scientific reports 10:216282020Saturated fatty acids induce insulin resistance in podocytes through inhibition of IRS1 via activation of both IKKβ and mTORC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenoit Denhez et. al.PubMed 33303821
- Frontiers in physiology 9:10512018Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Ge et. al.PubMed 30131712
- FEBS letters 591:1176-11862017The adaptors Grb10 and Grb14 are calmodulin-binding proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene García-Palmero et. al.PubMed 28295264
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GRB10 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13322
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
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Target data
Function
Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
Alternative names
GRBIR, KIAA0207, GRB10, Growth factor receptor-bound protein 10, GRB10 adapter protein, Insulin receptor-binding protein Grb-IR
Recommended products
Rabbit Polyclonal GRB10 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRB10 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GRB10 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13322
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Growth Factor Receptor-Bound Protein 10 often referred to as GRB10 is an adaptor protein with a mass of approximately 66 kDa. This protein interacts with various receptor tyrosine kinases and other intracellular signaling molecules. GRB10 is expressed in many tissues but it shows higher expression in the brain muscle and liver. It plays a significant role as a modulator in cellular processes that involve signal transduction.
- Biological function summary
GRB10 interacts with receptor tyrosine kinases such as the insulin receptor and engages in regulating the insulin signaling pathway. It acts as part of a complex that influences cell growth and metabolism. GRB10 binds Via its SH2 domain to activated receptors such as IGF-1R altering downstream signaling and having an impact on growth and metabolic processes.
- Pathways
GRB10 is integral to the PI3K-AKT signaling pathway and the mTOR pathway. It negatively regulates these pathways by binding to receptors and altering downstream effects. GRB10 interacts with proteins such as IRS1 and mTORC1 affecting how cells respond to insulin and growth factors. Through these interactions it contributes to cellular metabolism and growth regulation.
- Associated diseases and disorders
Studies show that GRB10 has connections to conditions like cancer and type 2 diabetes. Alterations in GRB10's function or expression can lead to abnormal cellular signaling contributing to oncogenesis due to disrupted growth signals. In type 2 diabetes GRB10 affects insulin signaling pathways which is related to insulin resistance. These disorders show interconnection with proteins like IRS1 where GRB10 modulates pathways influencing disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GRB10 antibody (ab154029)
7.5% SDS PAGE.
Secondary antibody - goat anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)
All lanes: Western blot - Anti-GRB10 antibody (ab154029) at 1/2000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa
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