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AB317371

Anti-GRB10 + GRB14 antibody

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Rabbit Polyclonal GRB10 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human GRB10 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GRBIR, KIAA0207, GRB10, Growth factor receptor-bound protein 10, GRB10 adapter protein, Insulin receptor-binding protein Grb-IR

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB10 + GRB14 Antibody (AB317371)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB10 + GRB14 Antibody (AB317371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue staining GRB10 + GRB14 using ab317371 at 1 : 400 overnight at 4degC, DAB staining. Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37degC for 30min.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GRB10 conjugated to Keyhole Limpet Haemocyanin.

Q13322

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 20% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRB10 and GRB14 also known as Growth Factor Receptor-Bound Protein 10 and 14 are adaptor proteins playing important roles in growth signaling pathways. GRB10 has a molecular mass of approximately 65 kDa while GRB14 is around 58 kDa. Both proteins interact with cell surface receptors and are expressed in several tissues including muscle brain and liver. By binding to these receptors they modulate the intracellular signaling dynamics affecting how cells respond to external growth signals.
Biological function summary

These proteins function as negative regulators of receptor tyrosine kinase signaling. They inhibit pathways activated by growth factors by binding directly to receptor sites preventing downstream activation. GRB10 and GRB14 are not parts of large complexes but serve more as connectors that influence signaling cascades. Their binding modulates insulin and other receptor signaling therefore playing an important role in cellular responses to growth stimuli.

Pathways

GRB10 and GRB14 are key components in both insulin and IGF (insulin-like growth factor) signaling pathways which are significant for metabolic regulation and growth. In these pathways they interact with others such as the insulin receptor substrate (IRS) proteins and can indirectly affect the MAPK/ERK pathway. Their regulatory action helps to maintain balance in cellular growth and division processes essential for normal cellular function.

GRB10 and GRB14 have links to metabolic conditions like Type 2 Diabetes and cancer. Their ability to modulate insulin signaling connects them to diabetes where improper function can lead to insulin resistance. Similarly by affecting growth factor pathways they are associated with cancer development as their dysregulation can lead to uncontrolled cell proliferation. In these diseases they interact with proteins such as the insulin receptor and components of the PI3K/AKT signaling cascade illuminating their integral role in disease progression and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms : interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
See full target information GRB10

Additional targets

Growth factor receptor-bound protein 14,Grb10,Grb14
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Product promise

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