AB183737

Anti-GRB7 antibody [EPR14099]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal GRB7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Growth factor receptor-bound protein 7, B47, Epidermal growth factor receptor GRB-7, GRB7 adapter protein, GRB7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR14099] (AB183737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR14099] (AB183737)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling GRB7 with purified ab183737 at 1/500 dilution (0.82 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-GRB7 antibody [EPR14099] (AB183737)

Blocking/Diluting buffer : 5% NFDM/TBST

The working pattern of ab183737 in SK-BR-3, T-47D and MCF7 is consistent with what has been described in PMID : 7907978 and 17916906

All lanes:

Western blot - Anti-GRB7 antibody [EPR14099] (ab183737) at 1/1000 dilution

Lane 1:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Carrier free

Anti-GRB7 antibody [EPR14099] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14099

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRB7 also known as Growth Factor Receptor-Bound Protein 7 is a signaling adapter protein with a molecular mass of approximately 55 kDa. It facilitates interactions between receptor tyrosine kinases and downstream signaling molecules. GRB7 is found in several tissues notably in breast and ovarian tissues. The protein plays a significant role in processes like cell migration and growth by mediating signal transduction pathways.

Biological function summary

Proteins like GRB7 interact with various cell surface receptors. GRB7 forms part of signaling complexes that modulate diverse cellular responses essential for cell communication and function. As an adapter protein GRB7 binds with SH2 and PH domains to facilitate signal transduction directly influencing cellular proliferation and movement. Its expression links with cell motility indicating its relevance in cellular responses such as migration and adhesion.

Pathways

Proteins influencing GRB7 highlight its involvement in several signaling pathways notably the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways play important roles in regulating cellular processes like growth survival and migration. GRB7 interacts closely with other proteins such as HER2 often amplifying the signaling related to growth factors further indicating its critical role in signal transduction networks.

GRB7 has implications in cancer and related abnormalities. In particular abnormal GRB7 expression is linked to breast cancer and ovarian cancer often associated with tumor progression and metastasis. It frequently associates with the HER2 protein a known player in aggressive cancer subtypes serving as a potential therapeutic target and prognostic marker. Understanding GRB7's involvement offers insights into new treatment strategies for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).

See full target information GRB7

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21163 PubMed38036593

2023

Unravelling transcriptomic complexity in breast cancer through modulation of DARPP-32 expression and signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Behnaz Saidy,Richa Vasan,Rosie Durant,Megan-Rose Greener,Adelynn Immanuel,Andrew R Green,Emad Rakha,Ian Ellis,Graham Ball,Stewart G Martin,Sarah J Storr

Oncotarget 11:2273-2289 PubMed32595827

2020

The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Anke Vermehren-Schmaedick,Paulette Mhawech-Fauceglia,Byung S Park,Tanja Pejovic,Shiuh-Wen Luoh

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRB7 antibody [EPR14099]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR14099] (AB183737)

Western blot - Anti-GRB7 antibody [EPR14099] (AB183737)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise