AB109618

Anti-GRB7 antibody [EPR4378]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal GRB7 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Growth factor receptor-bound protein 7, B47, Epidermal growth factor receptor GRB-7, GRB7 adapter protein, GRB7

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (AB109618)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (AB109618)

ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-GRB7 antibody [EPR4378] (AB109618)

All lanes:

Western blot - Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

Lane 1:

SKBR-3 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 60 kDa

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Lab

Western blot - Anti-GRB7 antibody [EPR4378] (AB109618)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID : 7907978 and 17916906.

All lanes:

Western blot - Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution

Lane 1:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

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Carrier free

Anti-GRB7 antibody [EPR4378] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4378

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Heat to 98°C, allow to cool for 10-20 minutes.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRB7 also known as Growth Factor Receptor-Bound Protein 7 is a signaling adapter protein with a molecular mass of approximately 55 kDa. It facilitates interactions between receptor tyrosine kinases and downstream signaling molecules. GRB7 is found in several tissues notably in breast and ovarian tissues. The protein plays a significant role in processes like cell migration and growth by mediating signal transduction pathways.

Biological function summary

Proteins like GRB7 interact with various cell surface receptors. GRB7 forms part of signaling complexes that modulate diverse cellular responses essential for cell communication and function. As an adapter protein GRB7 binds with SH2 and PH domains to facilitate signal transduction directly influencing cellular proliferation and movement. Its expression links with cell motility indicating its relevance in cellular responses such as migration and adhesion.

Pathways

Proteins influencing GRB7 highlight its involvement in several signaling pathways notably the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways play important roles in regulating cellular processes like growth survival and migration. GRB7 interacts closely with other proteins such as HER2 often amplifying the signaling related to growth factors further indicating its critical role in signal transduction networks.

GRB7 has implications in cancer and related abnormalities. In particular abnormal GRB7 expression is linked to breast cancer and ovarian cancer often associated with tumor progression and metastasis. It frequently associates with the HER2 protein a known player in aggressive cancer subtypes serving as a potential therapeutic target and prognostic marker. Understanding GRB7's involvement offers insights into new treatment strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).

See full target information GRB7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:1269-1289 PubMed39920335

2025

Haploinsufficient phenotypes promote selection of PTEN and ARID1A-deficient clones in human colon.

Applications

Unspecified application

Species

Unspecified reactive species

Nefeli Skoufou-Papoutsaki,Sam Adler,Shenay Mehmed,Claire Tume,Cora Olpe,Edward Morrissey,Richard Kemp,Anne-Claire Girard,Elisa B Moutin,Chandra Sekhar Reddy Chilamakuri,Jodi L Miller,Cecilia Lindskog,Fabian Werle,Kate Marks,Francesca Perrone,Matthias Zilbauer,David S Tourigny,Douglas J Winton

Communications biology 3:588 PubMed33067514

2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Atsumi,Hiroto Katoh,Daisuke Komura,Itaru Hashimoto,Genta Furuya,Hirotomo Koda,Hiroki Konishi,Ryohei Suzuki,Asami Yamamoto,Satsuki Yuba,Hiroyuki Abe,Yasushi Rino,Takashi Oshima,Tetsuo Ushiku,Masashi Fukayama,Yasuyuki Seto,Shumpei Ishikawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRB7 antibody [EPR4378]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (AB109618)

Western blot - Anti-GRB7 antibody [EPR4378] (AB109618)

Western blot - Anti-GRB7 antibody [EPR4378] (AB109618)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise