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Rabbit Recombinant Monoclonal GRB7 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-GRB7 antibody [EPR4378] (AB109618), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (AB109618), expandable thumbnail
  • Western blot - Anti-GRB7 antibody [EPR4378] (AB109618), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Expected
Rat
Predicted
Not recommended
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Heat to 98°C, allow to cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200 - 1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).

Alternative names

Growth factor receptor-bound protein 7, B47, Epidermal growth factor receptor GRB-7, GRB7 adapter protein, GRB7

Recommended products

Rabbit Recombinant Monoclonal GRB7 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4378
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GRB7 also known as Growth Factor Receptor-Bound Protein 7 is a signaling adapter protein with a molecular mass of approximately 55 kDa. It facilitates interactions between receptor tyrosine kinases and downstream signaling molecules. GRB7 is found in several tissues notably in breast and ovarian tissues. The protein plays a significant role in processes like cell migration and growth by mediating signal transduction pathways.

Biological function summary

Proteins like GRB7 interact with various cell surface receptors. GRB7 forms part of signaling complexes that modulate diverse cellular responses essential for cell communication and function. As an adapter protein GRB7 binds with SH2 and PH domains to facilitate signal transduction directly influencing cellular proliferation and movement. Its expression links with cell motility indicating its relevance in cellular responses such as migration and adhesion.

Pathways

Proteins influencing GRB7 highlight its involvement in several signaling pathways notably the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways play important roles in regulating cellular processes like growth survival and migration. GRB7 interacts closely with other proteins such as HER2 often amplifying the signaling related to growth factors further indicating its critical role in signal transduction networks.

Associated diseases and disorders

GRB7 has implications in cancer and related abnormalities. In particular abnormal GRB7 expression is linked to breast cancer and ovarian cancer often associated with tumor progression and metastasis. It frequently associates with the HER2 protein a known player in aggressive cancer subtypes serving as a potential therapeutic target and prognostic marker. Understanding GRB7's involvement offers insights into new treatment strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618), expandable thumbnail

    Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)

    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

    The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.

    All lanes: Western blot - Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution

    Lane 1: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 4: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)

    ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618), expandable thumbnail

    Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)

    All lanes: Western blot - Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

    Lane 1: SKBR-3 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 60 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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