Anti-GREB1 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (AB72999)

ICC/IF image of ab72999 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (AB72999)

IHC image of GREB1 antibody staining in a section of formalin-fixed paraffin-embedded normal human prostate performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab72999, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Unknown

Western blot - Anti-GREB1 antibody (AB72999)

All lanes:

Western blot - Anti-GREB1 antibody (ab72999) at 1 µg/mL

All lanes:

Human Prostate Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 216 kDa

Observed band size: 216 kDa

true

Exposure time: 2min