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AB326977

Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture)

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Rabbit Recombinant Multiclonal Gremlin 1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CKTSF1B1, DAND2, DRM, PIG2, GREM1, Gremlin-1, Cell proliferation-inducing gene 2 protein, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2, IHG-2

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Sandwich ELISA - Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture) (AB326977)
  • sELISA

Lab

Sandwich ELISA - Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture) (AB326977)

Interpolated concentration of native Gremlin 1 was measured at different sample concentrations. Undiluted sample is as follows : IMR-90 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture) (AB326977)
  • sELISA

Lab

Sandwich ELISA - Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture) (AB326977)

Interpolated concentration of native Gremlin 1 was measured at different sample concentrations. Undiluted samples are as follows : IMR-90 cell extract (0.5 mg/mL) and MRC-5 cell extract (0.5 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture) (AB326977)
  • sELISA

Lab

Sandwich ELISA - Anti-Gremlin 1 antibody [EPR29485-M1] - BSA and Azide free (Capture) (AB326977)

Sandwich ELISA with the capture antibody ab326977 dilution at 2 µg/mL and detector antibody ab326978 dilution at 0.5 µg/mL after biotin conjugation.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

EPR29485-M1

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab326977 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Gremlin 1. The recommended pair for sandwich ELISA is:

Capture: ab326977, Human Gremlin 1 Capture Antibody (unconjugated)

Detector: ab326978, Human Gremlin 1 Detector Antibody (unconjugated)

The reference range value is 78.1-10000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner (By similarity). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (PubMed : 27036124). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed (By similarity).
See full target information GREM1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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