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AB251683

Anti-GRF2 antibody

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(2 Publications)

Rabbit Polyclonal GRF2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAPGEF1 aa 50-250.

View Alternative Names

GRF2, RAPGEF1, Rap guanine nucleotide exchange factor 1, CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)

Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for RAPGEF1 when incubated with ab251683 at a 1/50 dilution (negative control).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for RAPGEF1 with ab251683 at a 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-GRF2 antibody (AB251683)
  • WB

Unknown

Western blot - Anti-GRF2 antibody (AB251683)

All lanes:

Western blot - Anti-GRF2 antibody (ab251683) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

GRF2 overexpression HEK-293T cell lysate

Predicted band size: 121 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for RAPGEF1 with ab251683 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRF2 antibody (AB251683)

Formalin-fixed, paraffin-embedded human placenta tissue stained for RAPGEF1 with ab251683 at a 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RAPGEF1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q13905

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRF2 also known as GTP-binding protein GRF2 plays a mechanical role as a guanine nucleotide exchange factor (GEF) for the Rho family of small GTPases. It has a molecular mass of approximately 94 kDa. This protein assists in the exchange of GDP for GTP therefore activating Rho GTPases. GRF2 is highly expressed in brain tissue specifically in neurons indicating a role in neurological processes.
Biological function summary

GRF2 functions as a critical regulator of signal transduction pathways. It forms part of signaling complexes that transmit signals from receptors on the cell surface to intracellular effectors. Through its GEF activity GRF2 influences cytoskeletal reorganization which impacts cell morphology motility and growth. It interacts with other proteins such as ELMO1 to facilitate cellular responses to external stimuli.

Pathways

GRF2 integrates into the MAPK/ERK pathway amplifying signals that trigger cellular proliferation and survival. GRF2 also links to the Rho/ROCK pathway regulating actin dynamics and cellular architecture. Within these pathways GRF2 functions in concert with proteins such as Ras and RhoA ensuring effective transmission and modulation of signals that govern various cellular activities.

GRF2 has relevance to neurological and cancer-related conditions. In the context of neurological disorders alterations in GRF2 expression or function can contribute to the pathophysiology of conditions like schizophrenia. In cancer GRF2 may influence tumor progression linking to proteins such as Ras which is often mutated in various cancers. Understanding GRF2's role provides insight into its potential as a therapeutic target for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1 (PubMed : 12432078). Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
See full target information RAPGEF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:28 PubMed40045194

2025

tRF-5028c disrupts trophoblast function in recurrent spontaneous abortion by inhibiting CRKL-mediated Rap1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jialyu Huang,Jiawei Wang,Shuang Wang,Xiangpeng Xiong,Ruiyin Jiang,Chaoyi Xiong,Lu Wang,Lingling Huang,Yan Zhao,Zheng Fang,Xiaoyan Ai,Jiaying Lin

Neoplasia (New York, N.Y.) 44:100931 PubMed37647805

2023

Histone H3 K27M-mediated regulation of cancer cell stemness and differentiation in diffuse midline glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Sharma,Ivana Barravecchia,Brian Magnuson,Sarah F Ferris,April Apfelbaum,Nneka E Mbah,Jeanette Cruz,Varunkumar Krishnamoorthy,Robert Teis,McKenzie Kauss,Carl Koschmann,Costas A Lyssiotis,Mats Ljungman,Stefanie Galban
View all publications
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