AB221058

Anti-GRHL3 antibody

Name: Anti-GRHL3 antibody (ab221058) | Abcam
Brand: Abcam Limited
SKU: AB221058
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal GRHL3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL3 aa 50 to C-terminus.

View Alternative Names

SOM, TFCP2L4, GRHL3, Grainyhead-like protein 3 homolog, Sister of mammalian grainyhead, Transcription factor CP2-like 4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemical analysis of human skeletal muscle tissue labeling GRHL3 with ab221058 at a 1/1000 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemical analysis of human skin tissue labeling GRHL3 in the nucleus of squamous epithelial cells with ab221058 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemical analysis of human urinary bladder tissue labeling GRHL3 in the nucleus of squamous epithelial cells with ab221058 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemical analysis of human oral mucosa tissue labeling GRHL3 in the nucleus of squamous epithelial cells with ab221058 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-GRHL3 antibody (AB221058)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRHL3 antibody (AB221058)

Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling GRHL3 (green) in the nucleoplasm with ab221058 at 2 μg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GRHL3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TE85

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRHL3 also known as Grainyhead-like 3 functions mainly as a transcription factor. This protein weighs approximately 68 kDa and manages gene expression by binding to specific DNA sequences. GRHL3 is essential for keratinocyte differentiation and maintains epidermal integrity. The gene encoding GRHL3 shows expression mainly in epithelial tissues such as skin lungs and bladder.

Biological function summary

Grainyhead-like 3 coordinates the development and maintenance of epithelial tissues. It plays a significant role in wound healing by promoting cell migration and adhesion. GRHL3 forms part of a regulatory complex interacting with other transcription factors to control target genes involved in skin barrier formation. Besides its regulation extends to processes like epithelial-mesenchymal transition.

Pathways

The involvement of GRHL3 is important in the epidermal growth factor receptor (EGFR) signaling pathway and the Wnt signaling pathway. These pathways allow GRHL3 to influence cell proliferation and differentiation. The protein interacts with others like EGFR and beta-catenin in these contexts which shows its importance in skin physiology and development.

GRHL3 is associated with skin-related conditions including psoriasis and non-melanoma skin cancer. Mutations or dysregulation in GRHL3 expression link to aberrant skin cell proliferation and differentiation. In these contexts GRHL3 connections to proteins such as p53 and NF-kB have been identified indicating potential pathways for therapeutic targeting and intervention strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed : 21081122, PubMed : 25347468). xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Also required for proper development of the oral periderm (PubMed : 24360809). No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity (PubMed : 21081122).

See full target information GRHL3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2022:9235837 PubMed36246971

2022

SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongbo Du,Xiaobin Chen,Pingyu Zhu,Wei Sun,Qi Lv,Shulin Cheng,Xuesong Yang,Xiaodong Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRHL3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRHL3 antibody (AB221058)

Immunocytochemistry/ Immunofluorescence - Anti-GRHL3 antibody (AB221058)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise