JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB228921

Anti-GRHL3 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal GRHL3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL3.

View Alternative Names

SOM, TFCP2L4, GRHL3, Grainyhead-like protein 3 homolog, Sister of mammalian grainyhead, Transcription factor CP2-like 4

1 Images
Western blot - Anti-GRHL3 antibody (AB228921)
  • WB

Supplier Data

Western blot - Anti-GRHL3 antibody (AB228921)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-GRHL3 antibody (ab228921) at 1/5000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 59 kDa,65 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human GRHL3. The exact immunogen used to generate this antibody is proprietary information.

Q8TE85

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000.00000 - 1/10000.00000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRHL3 also known as Grainyhead-like 3 functions mainly as a transcription factor. This protein weighs approximately 68 kDa and manages gene expression by binding to specific DNA sequences. GRHL3 is essential for keratinocyte differentiation and maintains epidermal integrity. The gene encoding GRHL3 shows expression mainly in epithelial tissues such as skin lungs and bladder.
Biological function summary

Grainyhead-like 3 coordinates the development and maintenance of epithelial tissues. It plays a significant role in wound healing by promoting cell migration and adhesion. GRHL3 forms part of a regulatory complex interacting with other transcription factors to control target genes involved in skin barrier formation. Besides its regulation extends to processes like epithelial-mesenchymal transition.

Pathways

The involvement of GRHL3 is important in the epidermal growth factor receptor (EGFR) signaling pathway and the Wnt signaling pathway. These pathways allow GRHL3 to influence cell proliferation and differentiation. The protein interacts with others like EGFR and beta-catenin in these contexts which shows its importance in skin physiology and development.

GRHL3 is associated with skin-related conditions including psoriasis and non-melanoma skin cancer. Mutations or dysregulation in GRHL3 expression link to aberrant skin cell proliferation and differentiation. In these contexts GRHL3 connections to proteins such as p53 and NF-kB have been identified indicating potential pathways for therapeutic targeting and intervention strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed : 21081122, PubMed : 25347468). xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Also required for proper development of the oral periderm (PubMed : 24360809). No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity (PubMed : 21081122).
See full target information GRHL3
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB221058

Anti-GRHL3 antibody

primary-antibodies

grhl3-antibody-ab221058

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com