Rabbit Polyclonal GRHL3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL3.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GRHL3. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TE85
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
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Target data
Function
Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed:21081122, PubMed:25347468). xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Also required for proper development of the oral periderm (PubMed:24360809). No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity (PubMed:21081122).
Alternative names
SOM, TFCP2L4, GRHL3, Grainyhead-like protein 3 homolog, Sister of mammalian grainyhead, Transcription factor CP2-like 4
Recommended products
Rabbit Polyclonal GRHL3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GRHL3. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TE85
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRHL3 also known as Grainyhead-like 3 functions mainly as a transcription factor. This protein weighs approximately 68 kDa and manages gene expression by binding to specific DNA sequences. GRHL3 is essential for keratinocyte differentiation and maintains epidermal integrity. The gene encoding GRHL3 shows expression mainly in epithelial tissues such as skin lungs and bladder.
- Biological function summary
Grainyhead-like 3 coordinates the development and maintenance of epithelial tissues. It plays a significant role in wound healing by promoting cell migration and adhesion. GRHL3 forms part of a regulatory complex interacting with other transcription factors to control target genes involved in skin barrier formation. Besides its regulation extends to processes like epithelial-mesenchymal transition.
- Pathways
The involvement of GRHL3 is important in the epidermal growth factor receptor (EGFR) signaling pathway and the Wnt signaling pathway. These pathways allow GRHL3 to influence cell proliferation and differentiation. The protein interacts with others like EGFR and beta-catenin in these contexts which shows its importance in skin physiology and development.
- Associated diseases and disorders
GRHL3 is associated with skin-related conditions including psoriasis and non-melanoma skin cancer. Mutations or dysregulation in GRHL3 expression link to aberrant skin cell proliferation and differentiation. In these contexts GRHL3 connections to proteins such as p53 and NF-kB have been identified indicating potential pathways for therapeutic targeting and intervention strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GRHL3 antibody (ab228921)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-GRHL3 antibody (ab228921) at 1/5000 dilution
All lanes: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 59 kDa, 65 kDa, 70 kDa
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