AB251953

Anti-GRID2 antibody

Name: Anti-GRID2 antibody (ab251953) | Abcam
Brand: Abcam Limited
SKU: AB251953

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(2 Publications)

Rabbit Polyclonal GRID2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRID2 aa 650 to C-terminus.

View Alternative Names

GLURD2, GRID2, GluD2, GluR delta-2 subunit

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRID2 antibody (AB251953)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRID2 antibody (AB251953)

Paraffin-embedded human endometrium tissue stained for GRID2 using ab251953 at 1/50 dilution in immunohistochemical analysis. Low expression, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRID2 antibody (AB251953)

Paraffin-embedded human testis tissue stained for GRID2 using ab251953 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GRID2 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43424

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The gene symbol 'GRID2' encodes the protein Glutamate Receptor Ionotropic Delta-2 commonly called GRID2. It is part of the ionotropic glutamate receptor family and functions as ion channels in the central nervous system. GRID2 has an approximate mass of 110 kDa and is principally expressed in the cerebellum. This protein receptor plays a role in excitatory synaptic transmission by binding glutamate an essential neurotransmitter. It is not widespread in the body but has critical functions where it is present.

Biological function summary

The GRID2 protein is significant in synaptic plasticity and cerebellar functions by contributing to synapse formation and stability. It is part of the postsynaptic density (PSD) complex in neurons mainly affecting the communication between neuron cells. This protein helps in maintaining proper synaptic connections necessary for motor coordination and learning. The deficit of GRID2 can disrupt these functions affecting synaptic efficacy and neurological behavior.

Pathways

Grid ionotropic receptors like GRID2 play a pivotal role in the glutamatergic pathway and the synaptic signaling pathway. These pathways have strong associations with synaptic transmission and plasticity influencing learning and memory. The relationship of GRID2 with other proteins such as GRIA1 another glutamate receptor highlights its involvement in regulating synaptic strength and communication between neurons.

GRID2 mutations or dysfunctions link to neurological conditions such as spinocerebellar ataxia and autism spectrum disorders. Spinocerebellar ataxia patients show mutations that alter GRID2 function impacting motor coordination. In some autism spectrum disorder cases alterations in GRID2-supported pathways can influence synaptic connectivity which affects cognitive and social functioning. Connections with other proteins like GRIK2 suggest a network where GRID2 dysfunction contributes to these disease symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Member of the ionotropic glutamate receptor family, which plays a crucial role in synaptic organization and signal transduction in the central nervous system. Although it shares structural features with ionotropic glutamate receptors, does not bind glutamate as a primary ligand (PubMed : 34936451). Promotes synaptogenesis and mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis of cerebellar parallel fiber-Purkinje cell (PF-PC) synapses through the NRX1B-CBLN1-GRID2 triad complex (PubMed : 27418511). In the presence of neurexins and cerebellins, forms cation-selective channels that are proposed to be gated by glycine and D-serine (PubMed : 34936451). However, recent research disputes this ligand-gated cation channel activity (PubMed : 39052831). Cation-selective ion channel activity can be triggered by GRM1 in Purkinje cells (PubMed : 24357660, PubMed : 27276689).

See full target information GRID2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 32:104-121 PubMed35925837

2022

TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Qiyue Chen,Xin Wang,Shasha Zhao,Huan Deng,Baoqiang Guo,Cheng Zhang,Xiaoye Song,Wensheng Deng,Tongcun Zhang,Hongwei Ni

Acta neuropathologica 141:681-696 PubMed33609158

2021

Distinct amyloid-β and tau-associated microglia profiles in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Gerrits,Nieske Brouwer,Susanne M Kooistra,Maya E Woodbury,Yannick Vermeiren,Mirjam Lambourne,Jan Mulder,Markus Kummer,Thomas Möller,Knut Biber,Wilfred F A den Dunnen,Peter P De Deyn,Bart J L Eggen,Erik W G M Boddeke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GRID2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRID2 antibody (AB251953)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRID2 antibody (AB251953)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise