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AB251020

Anti-GRID2 antibody [EPR15423(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal GRID2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

GLURD2, GRID2, GluD2, GluR delta-2 subunit

2 Images
Western blot - Anti-GRID2 antibody [EPR15423(B)] - BSA and Azide free (AB251020)
  • WB

Supplier Data

Western blot - Anti-GRID2 antibody [EPR15423(B)] - BSA and Azide free (AB251020)

This data was developed using ab190358, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRID2 antibody [EPR15423(B)] (<a href='/en-us/products/primary-antibodies/grid2-antibody-epr15423b-ab190358'>ab190358</a>) at 1/10000 dilution

All lanes:

Human cerebellum at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa

false

Western blot - Anti-GRID2 antibody [EPR15423(B)] - BSA and Azide free (AB251020)
  • WB

Supplier Data

Western blot - Anti-GRID2 antibody [EPR15423(B)] - BSA and Azide free (AB251020)

This data was developed using ab190358, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRID2 antibody [EPR15423(B)] (<a href='/en-us/products/primary-antibodies/grid2-antibody-epr15423b-ab190358'>ab190358</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15423(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251020 is the carrier-free version of ab190358.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The gene symbol 'GRID2' encodes the protein Glutamate Receptor Ionotropic Delta-2 commonly called GRID2. It is part of the ionotropic glutamate receptor family and functions as ion channels in the central nervous system. GRID2 has an approximate mass of 110 kDa and is principally expressed in the cerebellum. This protein receptor plays a role in excitatory synaptic transmission by binding glutamate an essential neurotransmitter. It is not widespread in the body but has critical functions where it is present.
Biological function summary

The GRID2 protein is significant in synaptic plasticity and cerebellar functions by contributing to synapse formation and stability. It is part of the postsynaptic density (PSD) complex in neurons mainly affecting the communication between neuron cells. This protein helps in maintaining proper synaptic connections necessary for motor coordination and learning. The deficit of GRID2 can disrupt these functions affecting synaptic efficacy and neurological behavior.

Pathways

Grid ionotropic receptors like GRID2 play a pivotal role in the glutamatergic pathway and the synaptic signaling pathway. These pathways have strong associations with synaptic transmission and plasticity influencing learning and memory. The relationship of GRID2 with other proteins such as GRIA1 another glutamate receptor highlights its involvement in regulating synaptic strength and communication between neurons.

GRID2 mutations or dysfunctions link to neurological conditions such as spinocerebellar ataxia and autism spectrum disorders. Spinocerebellar ataxia patients show mutations that alter GRID2 function impacting motor coordination. In some autism spectrum disorder cases alterations in GRID2-supported pathways can influence synaptic connectivity which affects cognitive and social functioning. Connections with other proteins like GRIK2 suggest a network where GRID2 dysfunction contributes to these disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Member of the ionotropic glutamate receptor family, which plays a crucial role in synaptic organization and signal transduction in the central nervous system. Although it shares structural features with ionotropic glutamate receptors, does not bind glutamate as a primary ligand (PubMed : 34936451). Promotes synaptogenesis and mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis of cerebellar parallel fiber-Purkinje cell (PF-PC) synapses through the NRX1B-CBLN1-GRID2 triad complex (PubMed : 27418511). In the presence of neurexins and cerebellins, forms cation-selective channels that are proposed to be gated by glycine and D-serine (PubMed : 34936451). However, recent research disputes this ligand-gated cation channel activity (PubMed : 39052831). Cation-selective ion channel activity can be triggered by GRM1 in Purkinje cells (PubMed : 24357660, PubMed : 27276689).
See full target information GRID2
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