AB259886

Anti-GRIK1+GRIK2 antibody [EPR23995-85]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal GluK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Mouse, Rat, Human samples.

View Alternative Names

GLUR5, GRIK1, GluK1, Excitatory amino acid receptor 3, Glutamate receptor 5, EAA3, GluR-5, GluR5, GLUR6, GRIK2, GluK2, Excitatory amino acid receptor 4, Glutamate receptor 6, EAA4, GluR-6, GluR6

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID : 12783878). The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labeling GRIK1+GRIK2 with ab259886 at 1/50 dilution (11.14 μg/mL), counterstained with Anti-MAP2 mouse monoclonal antibody (ab11267) at 1/500 dilution (4 μg/mL), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution (2 μg/mL), counterstained with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)/ (ab150120) at 1/1000 dilution (2 μg/mL). Confocal image showing cytoplasmic staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Negative control 1 : ab259886 at 1/50 dilution (11.14 μg/mL), followed by ab150120 1/1000 (2 μg/mL).

Negative control 2 : ab11267 1/500 (4 μg/mL), followed by ab150081 1/1000 (2 μg/ml).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum.The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND ^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse lung. The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebellum (PMID : 11427689). The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labeling GRIK1+GRIK2 with ab259886 at 1/50 dilution (11.14 μg/mL), counterstained with Anti-MAP2 mouse monoclonal antibody (ab11267) at 1/500 dilution (4 μg/mL), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution (2 μg/mL), counterstained with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)/ (ab150120) at 1/1000 dilution (2 μg/mL). Confocal image showing cytoplasmic staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Negative control 1 : ab259886 at 1/50 dilution (11.14 μg/mL), followed by ab150120 1/1000 (2 μg/mL).

Negative control 2 : ab11267 1/500 (4 μg/mL), followed by ab150081 1/1000 (2 μg/ml).

Lab

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Bands around 250kDa and above might be dimer and tetramer according to the Uniprot database.

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : Lane1 : 48 seconds

Lane2 : 10 seconds

Lane 3 : 26 seconds

All lanes:

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 109 kDa

false

Lab

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : Mouse lung (PMID : 15014126)

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 26 seconds

All lanes:

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886) at 1/1000 dilution

Lane 1:

Mouse hypothalamus tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 109 kDa

false

Lab

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886) at 1/1000 dilution

Lane 1:

Mouse GRIK1 recombinant protein at 0.0005 µg

Lane 2:

Mouse GRIK2 recombinant protein at 0.0005 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 109 kDa

false

Carrier free

Anti-GRIK1+GRIK2 antibody [EPR23995-85] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23995-85

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Recombinant fragment - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRIK1 and GRIK2 also known as GluK1 and GluK2 are subunits of kainate receptors. These receptors are ionotropic glutamate receptors that facilitate excitatory neurotransmission in the central nervous system. GRIK1 and GRIK2 proteins have a molecular mass of approximately 100 kDa. They are expressed not only in the brain but also in other tissues though their expression is most abundantly observed in the hippocampus and cerebellum. These proteins play a role in modulating synaptic transmission and plasticity contributing to the overall functioning of neural circuits.

Biological function summary

Kainate receptors which include GRIK1 and GRIK2 form ion channels upon ligand binding allowing the flow of cations such as sodium and calcium across the cell membrane. These interactions significantly influence synaptic strength and neuronal communication. GRIK1 and GRIK2 often assemble in a tetrameric complex which may also include other subunits like GRIK3 enhancing the diversity of receptor functions. The activity of these receptors has a profound impact on neurodevelopment and cognitive processes.

Pathways

GRIK1 and GRIK2 play a pivotal role in glutamatergic signaling pathways specifically impacting synaptic plasticity and long-term potentiation (LTP). These pathways are critical for learning and memory formation. The kainate receptor functions alongside AMPA and NMDA receptors key participants in synaptic transmission. The collaborative interactions among these receptor types facilitate complex neuronal network activities. This involvement positions GRIK1 and GRIK2 as essential players in mediating excitatory neurotransmission that influences higher cognitive functions.

Dysregulation of GRIK1 and GRIK2 is linked with several neurological conditions including epilepsy and schizophrenia. Altered expression or function of kainate receptors can lead to imbalances in excitatory and inhibitory signaling contributing to epileptic seizures. In schizophrenia abnormalities in glutamate receptor signaling including that of GRIK1 and GRIK2 may affect cognition and perception. Additionally their interaction with proteins like NMDA receptor subunits further highlights their role in neuropsychiatric disorders. Understanding these connections can aid in the development of targeted therapeutic approaches.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.. Isoform 2. Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid.

See full target information GRIK1

Additional targets

GRIK2

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRIK1+GRIK2 antibody [EPR23995-85]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise