Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)

Rabbit Recombinant Monoclonal GluK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Mouse, Rat, Human samples.

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Images

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (AB259886), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	ICC/IF	IHC-Fr	IHC-P
Human	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Tested
Mouse	Not recommended	Not recommended	Tested	Tested	Not recommended	Tested
Rat	Not recommended	Not recommended	Tested	Tested	Not recommended	Tested
Recombinant fragment - Mouse	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -
Species Recombinant fragment - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -
Species Recombinant fragment - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Mouse	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Rat	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Mouse	Dilution info -	Notes -

Target data

See full target information GRIK1

Function

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. Isoform 2. Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid.

Additional Targets

GRIK2, GRIK2

Alternative names

GLUR5, GRIK1, GluK1, Excitatory amino acid receptor 3, Glutamate receptor 5, EAA3, GluR-5, GluR5

Recommended products

Rabbit Recombinant Monoclonal GluK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23995-85
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GRIK1 and GRIK2 also known as GluK1 and GluK2 are subunits of kainate receptors. These receptors are ionotropic glutamate receptors that facilitate excitatory neurotransmission in the central nervous system. GRIK1 and GRIK2 proteins have a molecular mass of approximately 100 kDa. They are expressed not only in the brain but also in other tissues though their expression is most abundantly observed in the hippocampus and cerebellum. These proteins play a role in modulating synaptic transmission and plasticity contributing to the overall functioning of neural circuits.

Biological function summary

Kainate receptors which include GRIK1 and GRIK2 form ion channels upon ligand binding allowing the flow of cations such as sodium and calcium across the cell membrane. These interactions significantly influence synaptic strength and neuronal communication. GRIK1 and GRIK2 often assemble in a tetrameric complex which may also include other subunits like GRIK3 enhancing the diversity of receptor functions. The activity of these receptors has a profound impact on neurodevelopment and cognitive processes.

Pathways

GRIK1 and GRIK2 play a pivotal role in glutamatergic signaling pathways specifically impacting synaptic plasticity and long-term potentiation (LTP). These pathways are critical for learning and memory formation. The kainate receptor functions alongside AMPA and NMDA receptors key participants in synaptic transmission. The collaborative interactions among these receptor types facilitate complex neuronal network activities. This involvement positions GRIK1 and GRIK2 as essential players in mediating excitatory neurotransmission that influences higher cognitive functions.

Associated diseases and disorders

Dysregulation of GRIK1 and GRIK2 is linked with several neurological conditions including epilepsy and schizophrenia. Altered expression or function of kainate receptors can lead to imbalances in excitatory and inhibitory signaling contributing to epileptic seizures. In schizophrenia abnormalities in glutamate receptor signaling including that of GRIK1 and GRIK2 may affect cognition and perception. Additionally their interaction with proteins like NMDA receptor subunits further highlights their role in neuropsychiatric disorders. Understanding these connections can aid in the development of targeted therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Mouse lung (PMID:15014126)
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 26 seconds
All lanes: Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886) at 1/1000 dilution
Lane 1: Mouse hypothalamus tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 109 kDa
Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Bands around 250kDa and above might be dimer and tetramer according to the Uniprot database.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: Lane1: 48 seconds
Lane2: 10 seconds
Lane 3: 26 seconds
All lanes: Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886) at 1/1000 dilution
Lane 1: Human hypothalamus tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Rat cerebellum tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 109 kDa
Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 5.5 seconds
All lanes: Western blot - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886) at 1/1000 dilution
Lane 1: Mouse GRIK1 recombinant protein at 0.0005 µg
Lane 2: Mouse GRIK2 recombinant protein at 0.0005 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 109 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebellum (PMID: 11427689). The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum.The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND ^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 12783878). The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labeling GRIK1+GRIK2 with ab259886 at 1/50 dilution (11.14 μg/mL), counterstained with Anti-MAP2 mouse monoclonal antibody (Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267) at 1/500 dilution (4 μg/mL), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution (2 μg/mL), counterstained with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)/ (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (2 μg/mL). Confocal image showing cytoplasmic staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Negative control 1: ab259886 at 1/50 dilution (11.14 μg/mL), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 1/1000 (2 μg/mL).
Negative control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 1/500 (4 μg/mL), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1/1000 (2 μg/ml).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling GRIK1+GRIK2 with ab259886 at 1/1000 (0.557 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse lung. The section was incubated with ab259886 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-GRIK1+GRIK2 antibody [EPR23995-85] (ab259886)
Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labeling GRIK1+GRIK2 with ab259886 at 1/50 dilution (11.14 μg/mL), counterstained with Anti-MAP2 mouse monoclonal antibody (Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267) at 1/500 dilution (4 μg/mL), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution (2 μg/mL), counterstained with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)/ (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (2 μg/mL). Confocal image showing cytoplasmic staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Negative control 1: ab259886 at 1/50 dilution (11.14 μg/mL), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 1/1000 (2 μg/mL).
Negative control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 1/500 (4 μg/mL), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1/1000 (2 μg/ml).