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AB66440

Anti-GRIK2/GluK2 antibody

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(2 Publications)

Rabbit Polyclonal GRIK2/GluK2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

GLUR6, GRIK2, GluK2, Excitatory amino acid receptor 4, Glutamate receptor 6, EAA4, GluR-6, GluR6

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRIK2/GluK2 antibody (AB66440)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRIK2/GluK2 antibody (AB66440)

ICC/IF image of ab66440 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66440, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GRIK2 protein also known as GluK2 is a subunit of the kainate receptor which is part of the ionotropic glutamate receptor family. This receptor primarily mediates excitatory synaptic transmission in the central nervous system. GRIK2 has a molecular weight of approximately 104 kDa and is highly expressed in regions such as the hippocampus cortex and cerebellum. These areas are critical for learning and memory processes which underlines the importance of GluK2 in neuronal communication.
Biological function summary

GRIK2 plays a significant role in modulating synaptic plasticity an important mechanism for learning and memory. It forms heteromeric complexes with other kainate receptor subunits like GluK1 and GluK3 which can affect receptor properties. This combination allows for diverse functional responses and adaptability in synaptic transmission. The presence of GRIK2 in neurons indicates that it is integral in processes such as synaptic strength modulation and response to different synaptic inputs.

Pathways

GRIK2 is essential in the glutamatergic signaling pathway which is involved in many excitatory transmissions within the brain. This protein interacts with associated proteins such as PSD-95 and PICK1 which helps in receptor clustering and synaptic signaling regulation. Additionally GRIK2 is involved in calcium signaling pathways through its ability to regulate calcium influx contributing to neuron function and communication.

GRIK2 has been linked to conditions such as epilepsy and schizophrenia. Mutations or dysregulation in the expression of GRIK2 can affect neurotransmission balance which can lead to these neurological disorders. Its interaction with other receptor subunits like GluK1 and GluK3 also connects GRIK2 to these diseases since alterations in the complex can disrupt normal synaptic function. Understanding these relationships is key in developing therapeutic strategies for such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid (PubMed : 7536611, PubMed : 8730589, PubMed : 14511640). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformational change leading to the opening of the cation channel, converting the chemical signal to an electrical impulse (By similarity). The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 14511640, PubMed : 28180184, PubMed : 34375587, PubMed : 7536611, PubMed : 8730589). Modulates cell surface expression of NETO2 (By similarity). In association with GRIK3, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (By similarity).. Independent of its ionotropic glutamate receptor activity, acts as a thermoreceptor conferring sensitivity to cold temperatures (PubMed : 31474366). Functions in dorsal root ganglion neurons (By similarity).
See full target information GRIK2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and molecular pathology 134:104874 PubMed37775022

2023

SARS-CoV-2 hampers dopamine production in iPSC-derived dopaminergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

G Cappelletti,E V Carsana,G Lunghi,S Breviario,C Vanetti,A B Di Fonzo,E Frattini,M Magni,S Zecchini,M Clerici,M Aureli,C Fenizia

Frontiers in cellular neuroscience 12:117 PubMed29766991

2018

Response: Commentary: Analysis of SUMO1-conjugation at synapses.

Applications

WB

Species

Unspecified reactive species

James A Daniel,Benjamin H Cooper,Jorma J Palvimo,Fu-Ping Zhang,Nils Brose,Marilyn Tirard
View all publications
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Product promise

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