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AB250648

Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] - BSA and Azide free

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Rabbit Recombinant Monoclonal GRIK3/GluK3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GLUR7, GRIK3, GluK3, Excitatory amino acid receptor 5, Glutamate receptor 7, EAA5, GluR-7, GluR7, GLUR6, GRIK2, GluK2, Excitatory amino acid receptor 4, Glutamate receptor 6, EAA4, GluR-6, GluR6

2 Images
Western blot - Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] - BSA and Azide free (AB250648)
  • WB

Supplier Data

Western blot - Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] - BSA and Azide free (AB250648)

This data was developed using ab183035, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] (<a href='/en-us/products/primary-antibodies/grik2-gluk2-grik3-gluk3-antibody-epr13726-ab183035'>ab183035</a>) at 1/1000 dilution

All lanes:

Human hippocampus cell lysate at 20 µg

Secondary

All lanes:

Gaot Anti-rabbit IgG HRP at 1/1000 dilution

Predicted band size: 104 kDa

false

Western blot - Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] - BSA and Azide free (AB250648)
  • WB

Supplier Data

Western blot - Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] - BSA and Azide free (AB250648)

This data was developed using ab183035, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726] (<a href='/en-us/products/primary-antibodies/grik2-gluk2-grik3-gluk3-antibody-epr13726-ab183035'>ab183035</a>) at 1/5000 dilution

All lanes:

Human cerebellum cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-rabbit IgG HRP at 1/1000 dilution

Predicted band size: 104 kDa

false

  • Unconjugated

    Anti-GRIK2/GluK2 + GRIK3/GluK3 antibody [EPR13726]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13726

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Biochemicals

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Reactivity data

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Product details

ab250648 is the carrier-free version of ab183035.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRIK2 and GRIK3 also known as GluK2 and GluK3 are ionotropic receptors that facilitate synaptic transmission in the central nervous system. They are part of the kainate receptor family which operates by binding the neurotransmitter glutamate. The molecular mass for GluK2 is approximately 98 kDa while GluK3 is similar in size. These receptors predominantly localize in the brain particularly in the hippocampus and cerebellum where they play significant roles in excitatory neurotransmission.
Biological function summary

GRIK2 and GRIK3 function as part of ligand-gated ion channels which regulate the flow of ions across the cell membrane upon activation by glutamate. They contribute to forming heteromeric receptor complexes with other kainate receptor subunits. Such complexes are critical in modulating synaptic plasticity and neuronal excitability influencing learning and memory processes.

Pathways

GRIK2 and GRIK3 engage in the glutamatergic synapse pathway. This pathway involves critical proteins like AMPA and NMDA receptors which also bind glutamate and contribute to synaptic transmission and plasticity. The receptors assist in maintaining the balance of excitation and inhibition in neuronal networks playing important roles in both normal physiology and response to neurotransmitter fluctuations.

GRIK2 and GRIK3 have associations with neurological conditions such as epilepsy and schizophrenia. Their altered function or expression can disrupt synaptic transmission leading to abnormal neuronal excitability and network dysfunction. They interact with NMDA receptors further affecting glutamatergic signaling in these diseases potentially serving as targets for therapeutic intervention strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 7719709). In association with GRIK2, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (By similarity).
See full target information GRIK3

Additional targets

GRIK2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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