AB233734

Anti-GRIK3/GluK3 antibody [2B3D1]

Name: Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) | Abcam
Brand: Abcam Limited
SKU: AB233734
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal GRIK3/GluK3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRIK3 aa 1-200.

View Alternative Names

GLUR7, GRIK3, GluK3, Excitatory amino acid receptor 5, Glutamate receptor 7, EAA5, GluR-7, GluR7

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Immunofluorescence analysis of Hela (human epithelial cell line from cervix adenocarcinoma) cells labelling GRIK3/GluK3 using ab233734 at 1/200 dilution. Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments labeled with AlexaFluor^®-555-Phalloidin.

Supplier Data

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Expected MW is 42 kDa.

All lanes:

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) at 1/500 dilution

All lanes:

Human GRIK3/GluK3 (aa 32-173) recombinant protein

Predicted band size: 104 kDa

false

Supplier Data

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

All lanes:

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.

Lane 2:

GRIK3/GluK3 (aa 32-173)-hIgGFc transfected HEK293 cell lysate.

Predicted band size: 104 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2B3D1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRIK3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q13003

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GRIK3 gene encodes the protein GluK3 also known as kainate receptor subunit 3. This protein is involved in mediating synaptic transmission in the central nervous system. GluK3 is part of the ionotropic glutamate receptor family which forms ligand-gated ion channels. The protein has a molecular mass of approximately 107 kDa. GRIK3 is expressed in several regions of the brain including the cortex hippocampus and cerebellum indicating its important roles in these areas.

Biological function summary

The ionotropic receptor family includes GluK3 which forms a functional protein complex known for mediating excitatory neurotransmission. The activity of GluK3 involves the binding of glutamate leading to the opening of cation channels and allowing the flow of sodium and potassium ions. This process results in the depolarization of neurons contributing to synaptic plasticity and excitatory synaptic activity in the brain. The precise functioning of GluK3 is important for maintaining normal synaptic transmission and neuronal communication.

Pathways

The GRIK3/GluK3 protein significantly contributes to the glutamatergic synapse pathway and influences the long-term potentiation (LTP) pathway. GluK3 plays a role in regulating synaptic strength and plasticity by modulating neurotransmitter release and postsynaptic responses. Throughout these pathways it interacts with proteins such as GRIA1 and GRIN2 which are other members of the glutamate receptor family. These interactions ensure proper signal transmission and synaptic adaptation throughout neural circuits.

GRIK3/GluK3 shows associations with psychiatric conditions such as schizophrenia and bipolar disorder. The dysregulation of GluK3 function can lead to aberrant glutamatergic signaling which is implicated in the pathophysiology of these mental health disorders. Furthermore altered GluK3 activity may impact proteins like GRIA2 affecting the overall balance of excitatory and inhibitory signaling in the brain. Understanding the role of GRIK3 in these diseases may help in developing therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 7719709). In association with GRIK2, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (By similarity).

See full target information GRIK3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31062 PubMed37357387

2023

Periodontal ligament-associated protein-1 knockout mice regulate the differentiation of osteoclasts and osteoblasts through TGF-β1/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Liu,Xiao Yan,Jing Guo,Hong An,Xingrui Li,Liying Yang,Xijiao Yu,Shu Li

American journal of cancer research 13:2066-2075 PubMed37293152

2023

GRIK3 deficiency promotes non-small cell lung cancer progression by the regulation of the UBE2C/CDK1/Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Zhu-Xiang Zhao,Bin-Kai Li,Zi-Wen Zhao

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GRIK3/GluK3 antibody [2B3D1]

Immunocytochemistry/ Immunofluorescence - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise