Skip to main content

Mouse Monoclonal GRIM19 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Cow, Rat samples. Cited in 59 publications.


Images

Western blot - Anti-GRIM19 antibody [6E1BH7] (AB110240), expandable thumbnail
  • Western blot - Anti-GRIM19 antibody [6E1BH7] (AB110240), expandable thumbnail
  • Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (AB110240), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (AB110240), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cow
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Cow, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Cow
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Cow, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed:27626371). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:27626371). Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes (PubMed:15753091).

Alternative names

GRIM19, CDA016, CGI-39, NDUFA13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, Cell death regulatory protein GRIM-19, Complex I-B16.6, Gene associated with retinoic and interferon-induced mortality 19 protein, NADH-ubiquinone oxidoreductase B16.6 subunit, CI-B16.6, GRIM-19, Gene associated with retinoic and IFN-induced mortality 19 protein

Recommended products

Mouse Monoclonal GRIM19 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Cow, Rat samples. Cited in 59 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
6E1BH7
Light chain type
kappa
Concentration
Loading...
Purification notes

ab110240 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GRIM19 also known as NDUFA13 is a small protein with a molecular mass of approximately 16 kDa. It expresses mainly in mitochondria where it forms an integral component of the mitochondrial respiratory chain complex I. GRIM19 plays an important role in the functioning of complex I by facilitating the assembly and stabilization of the complex enabling efficient electron transport and ATP synthesis.

Biological function summary

GRIM19 interacts with several complexes and proteins within the mitochondrial inner membrane. As a part of complex I also known as NADH:ubiquinone oxidoreductase it contributes to the initial step of mitochondrial oxidative phosphorylation. GRIM19 also participates in the regulation of apoptosis and its expression is influenced during cellular stress situations highlighting its significance in cellular survival and energy production.

Pathways

GRIM19 integrates closely with mitochondrial apoptosis and energy metabolism pathways. Its interactions within oxidative phosphorylation place it in concert with both the electron transport chain and the apoptosis regulatory network. GRIM19 shares associations with related proteins such as cytochrome c oxidase and other complex I subunits highlighting its essential contribution to these important biological processes.

Associated diseases and disorders

GRIM19 demonstrates links to various pathologies including cancer and mitochondrial diseases. Alterations in GRIM19 expression or function have been implicated in the progression of certain cancers such as colorectal and thyroid cancer where GRIM19's usual regulatory role in apoptosis can be disrupted. Additionally its involvement in mitochondrial disease connects to proteins like COX-A1 emphasizing the importance of maintaining functional complexes for cellular health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240), expandable thumbnail

    Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)

    Lanes 1-3: Merged signal (red and green). Green - ab110240 observed at 17 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 36 kDa.

    ab110240 GRIM19 antibody [6E1BH7] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NDUFA13 (GRIM19) knockout HeLa cell line ab265863 (knockout cell lysate Human NDUFA13 (GRIM19) knockout HeLa cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab110240 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: NDUFA13 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human NDUFA13 (GRIM19) knockout HeLa cell line (Human NDUFA13 (GRIM19) knockout HeLa cell line ab265863)

    Lane 3: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 17 kDa, 184 kDa, 28 kDa, 56 kDa, 62 kDa, 76 kDa

    Observed band size: 17 kDa, 184 kDa, 29 kDa, 56 kDa, 70 kDa, 75 kDa

  • Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240), expandable thumbnail

    Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)

    All lanes: Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/mL

    Lane 1: Human heart mitochondria

    Lane 2: Bovine heart mitochondria

    Lane 3: Rat heart mitochondria

    Lane 4: Mouse heart mitochondria

    Predicted band size: 17 kDa

  • Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240), expandable thumbnail

    Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)

    HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)

    Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com