AB226201

Anti-GRIP1 antibody

Name: Anti-GRIP1 antibody (ab226201) | Abcam
Brand: Abcam Limited
SKU: AB226201
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal GRIP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GRIP1 aa 1000 to C-terminus.

View Alternative Names

Glutamate receptor-interacting protein 1, GRIP-1, GRIP1

2 Images

Supplier Data

Immunoprecipitation - Anti-GRIP1 antibody (AB226201)

GRIP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226201 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226201 at 1 μg/ml.

Lane 1 : ab226201 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-GRIP1 antibody (ab226201)

Predicted band size: 122 kDa

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Supplier Data

Western blot - Anti-GRIP1 antibody (AB226201)

All lanes:

Western blot - Anti-GRIP1 antibody (ab226201) at 0.4 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 122 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GRIP1 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3R0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226201 was affinity purified using an epitope specific to GRIP1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRIP1 also known as Glutamate Receptor Interacting Protein 1 is a protein that serves as a scaffold for various cellular signaling molecules. It weighs approximately 127 kDa. GRIP1 is expressed mainly in the central nervous system where it interacts with membrane proteins to facilitate their proper localization and function. It supports the clustering and stabilization of AMPA receptors at synaptic sites which is essential for synaptic transmission and plasticity.

Biological function summary

GRIP1 plays a role in synaptic formation and maintenance. It is a part of a multi-protein complex that influences the trafficking and stabilization of AMPA-type glutamate receptors. By regulating these receptors it contributes to synaptic plasticity a process fundamental for learning and memory. Additionally GRIP1 interacts with proteins like GRASP and liprin-α which further regulate synaptic density and neural connectivity.

Pathways

GRIP1 is involved in synaptic signaling pathways and neuronal communication. It participates in the maintenance of long-term potentiation (LTP) an activity-dependent strengthening of synapses that is central to memory and learning. GRIP1 interacts closely with proteins like GRASP1 and PICK1 in these pathways. These interactions are critical for mediating changes in synaptic strength and receptor turnover that underpin neural plasticity.

GRIP1's dysregulation associates with neuropsychiatric conditions such as autism spectrum disorder (ASD) and schizophrenia. Alterations in GRIP1 function can affect AMPA receptor trafficking impacting synaptic efficiency and cognitive function. In ASD GRIP1 connects with the protein SHANK3 influencing synapse functionality and neuronal development. In schizophrenia irregularities in GRIP1 contribute to synaptic dysfunction linking it to a broader network of proteins impacting glutamate signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons (PubMed : 10197531). Through complex formation with NSG1, GRIA2 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).

See full target information GRIP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:9070-9085 PubMed35293280

2022

Circular RNA circ_0002360 regulates the Taxol resistance and malignant behaviors of Taxol-resistant non-small cell lung cancer cells by microRNA-585-3p-dependent modulation of G protein regulated inducer of neurite outgrowth 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohai Cui,Boxiang Zhang,Baocheng Li,Xinju Li

PLoS pathogens 17:e1009812 PubMed34343212

2021

Mouse papillomavirus type 1 (MmuPV1) DNA is frequently integrated in benign tumors by microhomology-mediated end-joining.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Yu,Vladimir Majerciak,Xiang-Yang Xue,Aayushi Uberoi,Alexei Lobanov,Xiongfong Chen,Maggie Cam,Stephen H Hughes,Paul F Lambert,Zhi-Ming Zheng

Cell death discovery 7:164 PubMed34226511

2021

The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases.

Applications

Unspecified application

Species

Unspecified reactive species

María Del Carmen Conde-Rubio,Roman Mylonas,Christian Widmann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRIP1 antibody

Immunoprecipitation - Anti-GRIP1 antibody (AB226201)

Western blot - Anti-GRIP1 antibody (AB226201)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise