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AB108502

Anti-GRK1 antibody [EPR2039(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal GRK1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

RHOK, GRK1, Rhodopsin kinase GRK1, RK, G protein-coupled receptor kinase 1

1 Images
Western blot - Anti-GRK1 antibody [EPR2039(2)] (AB108502)
  • WB

Unknown

Western blot - Anti-GRK1 antibody [EPR2039(2)] (AB108502)

All lanes:

Western blot - Anti-GRK1 antibody [EPR2039(2)] (ab108502) at 1/1000 dilution

Lane 1:

Y79 cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Predicted band size: 63 kDa

Observed band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2039(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRK1 also known as G-protein-coupled receptor kinase 1 or rhodopsin kinase is an enzyme with a molecular mass of approximately 63 kDa. It plays a critical role in the phosphorylation process of activated rhodopsin. This kinase is mainly expressed in the retina particularly in rod photoreceptor cells where it aids in the regulation of phototransduction by rapidly shutting off the rhodopsin signaling cycle.
Biological function summary

GRK1 functions as part of a coordinated mechanism that ensures proper desensitization and recovery of photoreceptors after they are exposed to light. It is instrumental in the phototransduction cascade preventing excessive activation by phosphorylating light-activated rhodopsin which then allows the binding of arrestin. This phosphorylation-arrestin binding step is part of a larger complex that maintains the sensitivity of photoreceptors to changing light conditions.

Pathways

GRK1 participates in the critical visual signal transduction pathway within the retina. It works in conjunction with proteins such as arrestin and rhodopsin to modulate the phototransduction cascade ensuring proper visual responses. This kinase interacts with these proteins to modulate their activity and help in adapting to varying light conditions emphasizing its importance in maintaining visual clarity.

GRK1 mutations are connected to conditions like Oguchi disease a type of congenital stationary night blindness. This disease arises when rhodopsin phosphorylation is disrupted leading to impaired vision adaptation in low light. GRK1 links to the arrestin protein in this context as both proteins are necessary for proper rod cell function and dysfunction in either can lead to similar visual disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed : 15946941). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
See full target information GRK1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 6:148 PubMed33850109

2021

GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Pu,Ya Song,Mengdi Zhang,Caifeng Long,Jie Li,Yanan Wang,Yinzhe Xu,Fei Pan,Na Zhao,Xinyu Zhang,Yanan Xu,Jianxin Cui,Hongying Wang,Yan Li,Yong Zhao,Di Jin,Hongbing Zhang

JCI insight 3: PubMed29769445

2018

VEGF/VEGFR2 blockade does not cause retinal atrophy in AMD-relevant models.

Applications

Unspecified application

Species

Unspecified reactive species

Da Long,Yogita Kanan,Jikui Shen,Sean F Hackett,Yuanyuan Liu,Zibran Hafiz,Mahmood Khan,Lili Lu,Peter A Campochiaro
View all publications
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Product promise

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