Anti-GRK2 antibody - C-terminal

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (AB137666)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labelling GRK2 (Green) with ab137666 at 1/100 dilution. Alpha-tubulin filaments were also labelled (Red).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (AB137666)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling GRK2 protein at cytoplasm using ab137666 at a 1/1000 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (AB137666)

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft labelling GRK2 with ab137666 at 1/500 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-GRK2 antibody - C-terminal (AB137666)

Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 µg of GRK2 antibody [C2C3], C-term. Western blot analysis was performed using GRK2 antibody [C2C3], C-term.

All lanes:

Immunoprecipitation - Anti-GRK2 antibody - C-terminal (ab137666) at 5 µg

Lane 1:

Jurkat whole cell extracts with control IgG

Lane 2:

Jurkat whole cell extracts

Secondary

All lanes:

EasyBlot HRP-conjugated anti rabbit IgG antibody

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-GRK2 antibody - C-terminal (AB137666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-GRK2 antibody - C-terminal (AB137666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-GRK2 antibody - C-terminal (AB137666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution

Lane 1:

Wild-type GRK2 293T cell extracts at 30 µg

Lane 2:

GRK2 knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-GRK2 antibody - C-terminal (AB137666)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false