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AB308172

Anti-GRK2 antibody [HL1562]

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Rabbit Recombinant Monoclonal GRK2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Transfected cell lysate, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human GRK2.

View Alternative Names

ADRBK1, BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody [HL1562] (AB308172)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody [HL1562] (AB308172)

For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 10 min and permeabilized for detection of GRK2 protein using ab308172 at 1/500 dilution (green)

Nuclei (blue) is stained with fluoroshield with DAPI

Alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 (red)

Scale bar= 10μm

Immunohistochemistry paraffin embedded sections - Anti-GRK2 antibody [HL1562] (AB308172)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-GRK2 antibody [HL1562] (AB308172)

Immunohistochemical analysis of paraffin-embedded rat lymph nodes (left) and spleen (right) labeling GRK2 protein using ab308172 at 1/100 dilution. Heat mediated antigen retrieval with citrate buffer pH 6.0 for 15 mins.

Western blot - Anti-GRK2 antibody [HL1562] (AB308172)
  • WB

Supplier Data

Western blot - Anti-GRK2 antibody [HL1562] (AB308172)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (ab308172) diluted at 1/10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GRK2 antibody [HL1562] (ab308172) at 1/10000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 80 kDa

false

Western blot - Anti-GRK2 antibody [HL1562] (AB308172)
  • WB

Supplier Data

Western blot - Anti-GRK2 antibody [HL1562] (AB308172)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (ab308172) diluted at 1/2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

All lanes:

Western blot - Anti-GRK2 antibody [HL1562] (ab308172) at 1/2000 dilution

Lane 1:

Jurkat cell lysate at 30 µg

Lane 2:

Raji cell lysate at 30 µg

Lane 3:

NCI-H929 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 80 kDa

false

Western blot - Anti-GRK2 antibody [HL1562] (AB308172)
  • WB

Supplier Data

Western blot - Anti-GRK2 antibody [HL1562] (AB308172)

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (ab308172) diluted at 1/2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GRK2 antibody [HL1562] (ab308172) at 1/2000 dilution

Lane 1:

Mouse muscle tissue lysate at 50 µg

Lane 2:

Mouse small intestine tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1562

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GRK2. The exact immunogen used to generate this antibody is proprietary information.

P25098

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GRK2 also known as G protein-coupled receptor kinase 2 is an important enzyme involved in the regulation of G protein-coupled receptors (GPCRs). It has a molecular weight of approximately 80 kilodaltons. GRK2 is widely expressed in many tissues especially in the heart and brain where it plays an important role in cellular signaling processes. Its primary action is phosphorylating activated GPCRs which leads to receptor desensitization and downregulation modulating cellular responses to stimuli.
Biological function summary

GRK2 acts by coordinating with other proteins to regulate signal transduction pathways. It usually forms a complex with arrestins which prevent further signaling by blocking G protein coupling and directing receptors towards internalization pathways. This kinase acts to modulate receptor activity and maintain cellular homeostasis. GRK2 is essential for preventing overstimulation of cells playing a role in neurobiology and cardiology.

Pathways

GRK2 plays an important role in the adrenergic receptor signaling pathways including the beta-adrenergic and alpha-adrenergic pathways. It participates in the regulation of cardiac output and vascular tone by influencing the response of cells to catecholamines like adrenaline and noradrenaline. Proteins related to GRK2 in these pathways include adrenergic receptor subtypes and arrestin proteins. Voltage-gated calcium channels also interact with GRK2 linking it to calcium signaling pathways vital for muscle contraction and neurotransmitter release.

Research highlights GRK2's involvement in heart failure and hypertension. In heart failure elevated levels of GRK2 correlate with worsened cardiac function due to impaired beta-adrenergic receptor signaling. GRK2 is also linked to the development of hypertension through its effects on vascular smooth muscle cells and blood pressure regulation. Its interaction with proteins like arrestins suggests potential therapeutic targets for these cardiovascular conditions. Understanding and modulating GRK2 activity might offer approaches to treat these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed : 19715378). Phosphorylates catecholamine-activated ADRB2 to regulate physiological cardiomyocyte contraction rate responses (By similarity). Also phosphorylates ligand-bound C3a and C5a anaphylatoxin receptors (C3AR1 and C5AR1, respectively), leading to receptor desensitization (PubMed : 21799898, PubMed : 23077507). Key regulator of LPAR1 signaling (PubMed : 19306925). Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed : 19306925). Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed : 19306925). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity). Inhibits relaxation of airway smooth muscle in response to blue light (PubMed : 30284927).
See full target information GRK2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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