AB138438

Anti-GRK2 (phospho S685) antibody

Name: Anti-GRK2 (phospho S685) antibody (ab138438) | Abcam
Brand: Abcam Limited
SKU: AB138438
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal GRK2 phospho S685 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GRK2 phospho S685.

View Alternative Names

ADRBK1, BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2

1 Images

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Western blot - Anti-GRK2 (phospho S685) antibody (AB138438)

All lanes:

Western blot - Anti-GRK2 (phospho S685) antibody (ab138438) at 1/500 dilution

All lanes:

HT29 cells treated with 0.01 U/ml insulin for 15 min

Predicted band size: 80 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human GRK2 phospho S685. The exact immunogen used to generate this antibody is proprietary information.

P25098

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138438 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GRK2 also known as G protein-coupled receptor kinase 2 is an important enzyme involved in the regulation of G protein-coupled receptors (GPCRs). It has a molecular weight of approximately 80 kilodaltons. GRK2 is widely expressed in many tissues especially in the heart and brain where it plays an important role in cellular signaling processes. Its primary action is phosphorylating activated GPCRs which leads to receptor desensitization and downregulation modulating cellular responses to stimuli.

Biological function summary

GRK2 acts by coordinating with other proteins to regulate signal transduction pathways. It usually forms a complex with arrestins which prevent further signaling by blocking G protein coupling and directing receptors towards internalization pathways. This kinase acts to modulate receptor activity and maintain cellular homeostasis. GRK2 is essential for preventing overstimulation of cells playing a role in neurobiology and cardiology.

Pathways

GRK2 plays an important role in the adrenergic receptor signaling pathways including the beta-adrenergic and alpha-adrenergic pathways. It participates in the regulation of cardiac output and vascular tone by influencing the response of cells to catecholamines like adrenaline and noradrenaline. Proteins related to GRK2 in these pathways include adrenergic receptor subtypes and arrestin proteins. Voltage-gated calcium channels also interact with GRK2 linking it to calcium signaling pathways vital for muscle contraction and neurotransmitter release.

Research highlights GRK2's involvement in heart failure and hypertension. In heart failure elevated levels of GRK2 correlate with worsened cardiac function due to impaired beta-adrenergic receptor signaling. GRK2 is also linked to the development of hypertension through its effects on vascular smooth muscle cells and blood pressure regulation. Its interaction with proteins like arrestins suggests potential therapeutic targets for these cardiovascular conditions. Understanding and modulating GRK2 activity might offer approaches to treat these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed : 19715378). Key regulator of LPAR1 signaling (PubMed : 19306925). Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed : 19306925). Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed : 19306925). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity). Inhibits relaxation of airway smooth muscle in response to blue light (PubMed : 30284927).

See full target information GRK2 phospho S685

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 32:e22055 PubMed29697884

2018

Lophirones B and C halt acetaminophen hepatotoxicity by upregulating redox transcription factor Nrf-2 through Akt, PI3K, and PKC pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Najeeb O Aliyu,Rafiat A Ajala-Lawal,Taofeek O Ajiboye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRK2 (phospho S685) antibody

Western blot - Anti-GRK2 (phospho S685) antibody (AB138438)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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