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AB109303

Anti-GRK3 antibody [EPR2041(2)]

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(3 Publications)

Rabbit Recombinant Monoclonal GRK3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ADRBK2, BARK2, GRK3, G protein-coupled receptor kinase 3, Beta-adrenergic receptor kinase 2, Beta-ARK-2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (AB109303)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (AB109303)

ab109303, at 1/100 dilution, staining GRK3 in Human brain tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (AB109303)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (AB109303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling GRK3 with Purified ab109303 at 1 : 500 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-GRK3 antibody [EPR2041(2)] (AB109303)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRK3 antibody [EPR2041(2)] (AB109303)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GRK3 with Purified ab109303 at 1 : 50 dilution (4.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-GRK3 antibody [EPR2041(2)] (AB109303)
  • WB

Lab

Western blot - Anti-GRK3 antibody [EPR2041(2)] (AB109303)

All lanes:

Western blot - Anti-GRK3 antibody [EPR2041(2)] (ab109303) at 1/10000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 80 kDa

Observed band size: 75 kDa

false

  • Carrier free

    Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2041(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>For unpurified use at 1/100 - 1/250.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>For unpurified use at 1/100 - 1/250.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor kinase 3 (GRK3) also known as beta-adrenergic receptor kinase 2 (βARK2) is a serine/threonine kinase with approximate mass of 80 kDa. It mainly phosphorylates activated G protein-coupled receptors (GPCRs) to initiate receptor desensitization. GRK3 is expressed in various tissues including the brain heart and immune cells where it regulates cellular response to external signals. By phosphorylating GPCRs GRK3 facilitates binding of arrestin proteins which inhibits further signaling and contributes to receptor internalization.
Biological function summary

GRK3 influences signal transduction by modulating receptor sensitivity and turnover. It acts independently and as part of larger signaling complexes such as those involving β-arrestin proteins. This functional role affects numerous cellular processes including neurotransmitter release and immune responses. GRK3's activity and expression levels can modulate the intensity and duration of GPCR signaling affecting cellular intercommunication and adaptive responses.

Pathways

GRK3 plays a role in key signaling cascades like the adrenergic and chemokine pathways. It closely interacts with proteins like β-arrestin and GPCRs within these pathways guiding receptor trafficking and signal fidelity. The adrenergic pathway influences cardiovascular and central nervous system functions while the chemokine pathway impacts immune response and cell migration. By regulating these pathways GRK3 ensures balanced physiological and cellular responses.

GRK3 is linked to neurological disorders and cardiovascular diseases. Dysregulation of GRK3 affects receptor signaling disrupting processes like synaptic transmission and heart rate regulation. Altered GRK3 expression is associated with conditions like bipolar disorder where it influences mood regulation. GRK3 also connects to adrenergic receptors in the context of heart failure affecting cardiac performance and response to stress signals. Understanding GRK3's role offers insights for therapeutic strategies targeting these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

receptors (By similarity). Also phosphorylates ligand-bound C3a and C5a anaphylatoxin receptors (C3AR1 and C5AR1, respectively), leading to receptor desensitization (PubMed : 21799898).
See full target information GRK3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer letters 571:216333 PubMed37543278

2023

A novel GRK3-HDAC2 regulatory pathway is a key direct link between neuroendocrine differentiation and angiogenesis in prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Naderinezhad,Guoliang Zhang,Zheng Wang,Dayong Zheng,Mohit Hulsurkar,Michael Bakhoum,Ning Su,Han Yang,Tao Shen,Wenliang Li

Journal of experimental & clinical cancer research : CR 41:257 PubMed35996148

2022

GRK3 is a poor prognosticator and serves as a therapeutic target in advanced gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Yibo Fan,Jinbang Xu,Longfei Huo,Ailing W Scott,Jiankang Jin,Boxuan Yang,Shan Shao,Lang Ma,Ying Wang,Xiaodan Yao,Melissa Pool Pizzi,Matheus Sewastjanow Da Silva,Guoliang Zhang,Lijuan Zhuo,Eun Jeong Cho,Kevin N Dalby,Namita D Shanbhag,Zhenning Wang,Wenliang Li,Shumei Song,Jaffer A Ajani

British journal of pharmacology 178:3498-3516 PubMed33844281

2021

β-Arrestin1 and GPCR kinase2 play permissive roles in Src-mediated endocytosis of α4β2 nicotinic ACh receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Srijan Acharya,Dooti Kundu,Kyeong-Man Kim
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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