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Rabbit Polyclonal GRK3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Beta-adrenergic receptor kinase 2 aa 1-250.

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Images

Western blot - Anti-GRK3 antibody - N-terminal (AB137764), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody - N-terminal (AB137764), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Beta-adrenergic receptor kinase 2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P35626

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Target data

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Alternative names

Recommended products

Rabbit Polyclonal GRK3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Beta-adrenergic receptor kinase 2 aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Beta-adrenergic receptor kinase 2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P35626
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

G protein-coupled receptor kinase 3 (GRK3) also known as beta-adrenergic receptor kinase 2 (βARK2) is a serine/threonine kinase with approximate mass of 80 kDa. It mainly phosphorylates activated G protein-coupled receptors (GPCRs) to initiate receptor desensitization. GRK3 is expressed in various tissues including the brain heart and immune cells where it regulates cellular response to external signals. By phosphorylating GPCRs GRK3 facilitates binding of arrestin proteins which inhibits further signaling and contributes to receptor internalization.

Biological function summary

GRK3 influences signal transduction by modulating receptor sensitivity and turnover. It acts independently and as part of larger signaling complexes such as those involving β-arrestin proteins. This functional role affects numerous cellular processes including neurotransmitter release and immune responses. GRK3's activity and expression levels can modulate the intensity and duration of GPCR signaling affecting cellular intercommunication and adaptive responses.

Pathways

GRK3 plays a role in key signaling cascades like the adrenergic and chemokine pathways. It closely interacts with proteins like β-arrestin and GPCRs within these pathways guiding receptor trafficking and signal fidelity. The adrenergic pathway influences cardiovascular and central nervous system functions while the chemokine pathway impacts immune response and cell migration. By regulating these pathways GRK3 ensures balanced physiological and cellular responses.

Associated diseases and disorders

GRK3 is linked to neurological disorders and cardiovascular diseases. Dysregulation of GRK3 affects receptor signaling disrupting processes like synaptic transmission and heart rate regulation. Altered GRK3 expression is associated with conditions like bipolar disorder where it influences mood regulation. GRK3 also connects to adrenergic receptors in the context of heart failure affecting cardiac performance and response to stress signals. Understanding GRK3's role offers insights for therapeutic strategies targeting these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-GRK3 antibody - N-terminal (ab137764), expandable thumbnail

    Western blot - Anti-GRK3 antibody - N-terminal (ab137764)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-GRK3 antibody - N-terminal (ab137764) at 1/1000 dilution

    All lanes: HeLa whole cell lysate at 30 µg

    Predicted band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody - N-terminal (ab137764), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody - N-terminal (ab137764)

    Immunohistochemical analysis of paraffin-embedded Human HT1299 xenograft tissue, labelling GRK3 with ab137764 at 1/100 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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