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AB244364

Anti-GRK6 antibody

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(2 Publications)

Rabbit Polyclonal GRK6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRK6 aa 50-200.

View Alternative Names

GPRK6, GRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK6 antibody (AB244364)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK6 antibody (AB244364)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for GRK6 with ab244364 at a 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GRK6 antibody (AB244364)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRK6 antibody (AB244364)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling GRK6 using ab244364 at 4 μg/ml (green) in ICC/IF.

Western blot - Anti-GRK6 antibody (AB244364)
  • WB

Unknown

Western blot - Anti-GRK6 antibody (AB244364)

All lanes:

Western blot - Anti-GRK6 antibody (ab244364) at 0.4 µg/mL

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Human cell line MCF-7

Predicted band size: 65 kDa

false

Western blot - Anti-GRK6 antibody (AB244364)
  • WB

Unknown

Western blot - Anti-GRK6 antibody (AB244364)

All lanes:

Western blot - Anti-GRK6 antibody (ab244364) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

GRK6 overexpression HEK-293T lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRK6 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P43250

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor kinase 6 (GRK6) a member of the GRK family plays an important role in phosphorylating activated G protein-coupled receptors (GPCRs). GRK6 regulates receptor desensitization through this mechanism. It is also known as GPRK6. GRK6 has an approximate mass of 66 kDa. This kinase is expressed in various tissues including the brain heart and lymphoid organs contributing to its wide-ranging impact in physiological processes.
Biological function summary

GRK6 modulates signaling pathways by controlling the desensitization and internalization of GPCRs. The GRK6 protein localizes to the cellular membrane and operates as part of a larger signaling complex. This regulation process affects cellular responses like migration and immune reactions. GRK6 influences the reactivation and recycling of GPCRs impacting the duration and intensity of receptor-mediated signals in cells.

Pathways

GRK6 integrates into the GPCR signaling and chemokine signaling pathways. It directly modulates the activity of GPCRs contributing to cellular responses tied to these pathways. Related proteins such as beta-arrestins and G proteins also play significant roles in these interactions. GRK6 can phosphorylate GPCRs facilitating beta-arrestin binding which affects receptor trafficking and signaling.

GRK6 plays an emerging role in cancer pathogenesis and immune system disorders. Aberrant expression or function of GRK6 has been linked to chronic inflammatory diseases by altering immune cell signaling. Through its regulatory actions on GPCRs involved in inflammatory pathways GRK6 can connect to proteins such as chemokine receptors influencing the severity and progression of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor : LRP6 during Wnt signaling (in vitro).
See full target information GRK6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:278 PubMed40819077

2025

Targeting LINC02544/miR-497-5p/CAPRIN1 axis via exosome-based siRNA to overcome immunotherapy resistance in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Lian,Jiayi Li,Shihui Tang,Ting Li,Jinping Li

Genome research 34:2012-2024 PubMed38906680

2024

Long-read Ribo-STAMP simultaneously measures transcription and translation with isoform resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Pratibha Jagannatha,Alexandra T Tankka,Daniel A Lorenz,Tao Yu,Brian A Yee,Kristopher W Brannan,Cathy J Zhou,Jason G Underwood,Gene W Yeo
View all publications
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