Rabbit Recombinant Monoclonal GRK6 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-GRK6 antibody [EPR2046(2)] (AB109302), expandable thumbnail](https://content.abcam.com/products/images/grk6-antibody-epr20462-ab109302--western-blot-img123400.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GRK6 antibody [EPR2046(2)] (AB109302)")
Publications
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- British journal of pharmacology 178:3498-35162021β-Arrestin1 and GPCR kinase2 play permissive roles in Src-mediated endocytosis of α4β2 nicotinic ACh receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSrijan Acharya,Dooti Kundu,Kyeong-Man KimPubMed 33844281
- Molecular biology of the cell 24:1649-60, S1-32013Serine 129 phosphorylation of membrane-associated α-synuclein modulates dopamine transporter function in a G protein-coupled receptor kinase-dependent manner.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
Alternative names
GPRK6, GRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6
Recommended products
Rabbit Recombinant Monoclonal GRK6 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2046(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
G protein-coupled receptor kinase 6 (GRK6) a member of the GRK family plays an important role in phosphorylating activated G protein-coupled receptors (GPCRs). GRK6 regulates receptor desensitization through this mechanism. It is also known as GPRK6. GRK6 has an approximate mass of 66 kDa. This kinase is expressed in various tissues including the brain heart and lymphoid organs contributing to its wide-ranging impact in physiological processes.
- Biological function summary
GRK6 modulates signaling pathways by controlling the desensitization and internalization of GPCRs. The GRK6 protein localizes to the cellular membrane and operates as part of a larger signaling complex. This regulation process affects cellular responses like migration and immune reactions. GRK6 influences the reactivation and recycling of GPCRs impacting the duration and intensity of receptor-mediated signals in cells.
- Pathways
GRK6 integrates into the GPCR signaling and chemokine signaling pathways. It directly modulates the activity of GPCRs contributing to cellular responses tied to these pathways. Related proteins such as beta-arrestins and G proteins also play significant roles in these interactions. GRK6 can phosphorylate GPCRs facilitating beta-arrestin binding which affects receptor trafficking and signaling.
- Associated diseases and disorders
GRK6 plays an emerging role in cancer pathogenesis and immune system disorders. Aberrant expression or function of GRK6 has been linked to chronic inflammatory diseases by altering immune cell signaling. Through its regulatory actions on GPCRs involved in inflammatory pathways GRK6 can connect to proteins such as chemokine receptors influencing the severity and progression of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-GRK6 antibody [EPR2046(2)] (ab109302), expandable thumbnail](https://content.abcam.com/products/images/grk6-antibody-epr20462-ab109302--western-blot-img123400.jpg "Western blot - Anti-GRK6 antibody [EPR2046(2)] (ab109302)")
Western blot - Anti-GRK6 antibody [EPR2046(2)] (ab109302)
All lanes: Western blot - Anti-GRK6 antibody [EPR2046(2)] (ab109302) at 1/1000 dilution
Lane 1: U937 cell lysate at 10 µg
Lane 2: Ramos cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
Predicted band size: 65 kDa
Downloads
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