AB109302

Anti-GRK6 antibody [EPR2046(2)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal GRK6 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

GPRK6, GRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6

1 Images

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Western blot - Anti-GRK6 antibody [EPR2046(2)] (AB109302)

All lanes:

Western blot - Anti-GRK6 antibody [EPR2046(2)] (ab109302) at 1/1000 dilution

Lane 1:

U937 cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 65 kDa

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Carrier free

Anti-GRK6 antibody [EPR2046(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2046(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor kinase 6 (GRK6) a member of the GRK family plays an important role in phosphorylating activated G protein-coupled receptors (GPCRs). GRK6 regulates receptor desensitization through this mechanism. It is also known as GPRK6. GRK6 has an approximate mass of 66 kDa. This kinase is expressed in various tissues including the brain heart and lymphoid organs contributing to its wide-ranging impact in physiological processes.

Biological function summary

GRK6 modulates signaling pathways by controlling the desensitization and internalization of GPCRs. The GRK6 protein localizes to the cellular membrane and operates as part of a larger signaling complex. This regulation process affects cellular responses like migration and immune reactions. GRK6 influences the reactivation and recycling of GPCRs impacting the duration and intensity of receptor-mediated signals in cells.

Pathways

GRK6 integrates into the GPCR signaling and chemokine signaling pathways. It directly modulates the activity of GPCRs contributing to cellular responses tied to these pathways. Related proteins such as beta-arrestins and G proteins also play significant roles in these interactions. GRK6 can phosphorylate GPCRs facilitating beta-arrestin binding which affects receptor trafficking and signaling.

GRK6 plays an emerging role in cancer pathogenesis and immune system disorders. Aberrant expression or function of GRK6 has been linked to chronic inflammatory diseases by altering immune cell signaling. Through its regulatory actions on GPCRs involved in inflammatory pathways GRK6 can connect to proteins such as chemokine receptors influencing the severity and progression of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor : LRP6 during Wnt signaling (in vitro).

See full target information GRK6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 178:3498-3516 PubMed33844281

2021

β-Arrestin1 and GPCR kinase2 play permissive roles in Src-mediated endocytosis of α4β2 nicotinic ACh receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Srijan Acharya,Dooti Kundu,Kyeong-Man Kim

Molecular biology of the cell 24:1649-60, S1-3 PubMed23576548

2013

Serine 129 phosphorylation of membrane-associated α-synuclein modulates dopamine transporter function in a G protein-coupled receptor kinase-dependent manner.

Applications

Species

Human

Susumu Hara,Shigeki Arawaka,Hiroyasu Sato,Youhei Machiya,Can Cui,Asuka Sasaki,Shingo Koyama,Takeo Kato

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRK6 antibody [EPR2046(2)]

Western blot - Anti-GRK6 antibody [EPR2046(2)] (AB109302)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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