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AB247827

Anti-GRK6 antibody [EPR2046(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal GRK6 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GPRK6, GRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6

1 Images
Western blot - Anti-GRK6 antibody [EPR2046(2)] - BSA and Azide free (AB247827)
  • WB

Unknown

Western blot - Anti-GRK6 antibody [EPR2046(2)] - BSA and Azide free (AB247827)

This data was developed using ab109302, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRK6 antibody [EPR2046(2)] (<a href='/en-us/products/primary-antibodies/grk6-antibody-epr20462-ab109302'>ab109302</a>) at 1/1000 dilution

Lane 1:

U937 cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2046(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247827 is the carrier-free version of ab109302.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor kinase 6 (GRK6) a member of the GRK family plays an important role in phosphorylating activated G protein-coupled receptors (GPCRs). GRK6 regulates receptor desensitization through this mechanism. It is also known as GPRK6. GRK6 has an approximate mass of 66 kDa. This kinase is expressed in various tissues including the brain heart and lymphoid organs contributing to its wide-ranging impact in physiological processes.
Biological function summary

GRK6 modulates signaling pathways by controlling the desensitization and internalization of GPCRs. The GRK6 protein localizes to the cellular membrane and operates as part of a larger signaling complex. This regulation process affects cellular responses like migration and immune reactions. GRK6 influences the reactivation and recycling of GPCRs impacting the duration and intensity of receptor-mediated signals in cells.

Pathways

GRK6 integrates into the GPCR signaling and chemokine signaling pathways. It directly modulates the activity of GPCRs contributing to cellular responses tied to these pathways. Related proteins such as beta-arrestins and G proteins also play significant roles in these interactions. GRK6 can phosphorylate GPCRs facilitating beta-arrestin binding which affects receptor trafficking and signaling.

GRK6 plays an emerging role in cancer pathogenesis and immune system disorders. Aberrant expression or function of GRK6 has been linked to chronic inflammatory diseases by altering immune cell signaling. Through its regulatory actions on GPCRs involved in inflammatory pathways GRK6 can connect to proteins such as chemokine receptors influencing the severity and progression of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor : LRP6 during Wnt signaling (in vitro).
See full target information GRK6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com