Rabbit Polyclonal GRLF1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP35 aa 200-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGAP35 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRY4
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621).
Alternative names
GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP, ARHGAP35, Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, Rho GAP p190A, GRF-1, p190-A
Recommended products
Rabbit Polyclonal GRLF1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP35 aa 200-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGAP35 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRY4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRLF1 also known as Glucocorticoid Receptor DNA Binding Factor 1 is a protein with a mass of approximately 196 kDa. This protein plays a role in gene regulation by interacting with DNA binding sites associated with glucocorticoid receptors. GRLF1 is widely expressed in various tissues but shows a higher expression level in the brain. It functions in stabilizing the linkage between glucocorticoid receptors and chromatin facilitating important transcriptional processes.
- Biological function summary
GRLF1 interacts with nuclear receptors to regulate gene transcription and modulates cellular responses to glucocorticoids. It serves as a co-factor aiding in the assembly of transcriptional complexes needed for hormonal regulation. By forming complexes with other transcription factors GRLF1 influences cellular differentiation and immune response. Its involvement in the regulation of gene expression suggests a significant impact on cell signaling and homeostatic balance.
- Pathways
GRLF1 contributes to the glucocorticoid signaling pathway and the MAPK/ERK pathway. In the glucocorticoid pathway it cooperates with the GR protein family to adapt cellular responses to stress and inflammation. Within the MAPK/ERK pathway GRLF1 assists in regulating cell growth and differentiation by interacting with proteins like ERK1/2 impacting cell cycle progression.
- Associated diseases and disorders
Researchers link GRLF1 to neurological disorders and inflammatory conditions. In neurodegenerative diseases altered GRLF1 function may affect neuronal survival and cognitive function. Its dysregulation can also contribute to autoimmune disorders where the inflammatory response becomes imbalanced. Through these connections GRLF1 interacts with proteins such as GR and NF-kB influencing both normal physiology and pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-GRLF1 antibody (ab247123)
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling GRLF1 using ab247123 at 4 μg/ml (green) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRLF1 antibody (ab247123)
Paraffin-embedded human colon tissue stained for GRLF1 using ab247123 at 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRLF1 antibody (ab247123)
Paraffin-embedded human fallopian tube tissue stained for GRLF1 using ab247123 at 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRLF1 antibody (ab247123)
Paraffin-embedded human prostate tissue stained for GRLF1 using ab247123 at 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRLF1 antibody (ab247123)
Paraffin-embedded human testis tissue stained for GRLF1 using ab247123 at 1/20 dilution in immunohistochemical analysis.
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