AB85950

Anti-GRLF1 antibody

Name: Anti-GRLF1 antibody (ab85950) | Abcam
Brand: Abcam Limited
SKU: AB85950
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

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(1 Publication)

Rabbit Polyclonal GRLF1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARHGAP35 aa 1200-1250.

View Alternative Names

GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP, ARHGAP35, Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, Rho GAP p190A, GRF-1, p190-A

3 Images

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Immunoprecipitation - Anti-GRLF1 antibody (AB85950)

Detection of human GRLF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85950 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-GRLF1 antibody (ab85950)

Predicted band size: 172 kDa

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Supplier Data

Western blot - Anti-GRLF1 antibody (AB85950)

All lanes:

Western blot - Anti-GRLF1 antibody (ab85950) at 1/2000 dilution

Lane 1:

CT26-WT (Mouse colon carcinoma cell - Wild Type) whole cell lysate at 50 µg

Lane 2:

4T1 (Mouse mammary gland cells) whole cell lysate at 50 µg

Lane 3:

Renca (Mouse renal carcinoma cells) whole cell lysate at 50 µg

Predicted band size: 172 kDa

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Exposure time: 30s

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Western blot - Anti-GRLF1 antibody (AB85950)

All lanes:

Western blot - Anti-GRLF1 antibody (ab85950) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 172 kDa

Observed band size: 172 kDa

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Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ARHGAP35 aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information.

Q9NRY4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab85950 was affinity purified using an epitope specific to GRLF1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRLF1 also known as Glucocorticoid Receptor DNA Binding Factor 1 is a protein with a mass of approximately 196 kDa. This protein plays a role in gene regulation by interacting with DNA binding sites associated with glucocorticoid receptors. GRLF1 is widely expressed in various tissues but shows a higher expression level in the brain. It functions in stabilizing the linkage between glucocorticoid receptors and chromatin facilitating important transcriptional processes.

Biological function summary

GRLF1 interacts with nuclear receptors to regulate gene transcription and modulates cellular responses to glucocorticoids. It serves as a co-factor aiding in the assembly of transcriptional complexes needed for hormonal regulation. By forming complexes with other transcription factors GRLF1 influences cellular differentiation and immune response. Its involvement in the regulation of gene expression suggests a significant impact on cell signaling and homeostatic balance.

Pathways

GRLF1 contributes to the glucocorticoid signaling pathway and the MAPK/ERK pathway. In the glucocorticoid pathway it cooperates with the GR protein family to adapt cellular responses to stress and inflammation. Within the MAPK/ERK pathway GRLF1 assists in regulating cell growth and differentiation by interacting with proteins like ERK1/2 impacting cell cycle progression.

Researchers link GRLF1 to neurological disorders and inflammatory conditions. In neurodegenerative diseases altered GRLF1 function may affect neuronal survival and cognitive function. Its dysregulation can also contribute to autoimmune disorders where the inflammatory response becomes imbalanced. Through these connections GRLF1 interacts with proteins such as GR and NF-kB influencing both normal physiology and pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Rho GTPase-activating protein (GAP) (PubMed : 19673492, PubMed : 28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed : 19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed : 19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed : 1894621).

See full target information ARHGAP35

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:1385 PubMed32850446

2020

C1222C Deletion in Exon 8 of Is Involved in Carcinogenesis and Cell Cycle Control of Colorectal Cancer Through IRS1/PI3K/Akt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Jian Cao,Ya-Ning Zhu,Yu Ma,Ghulam Murtaza,Yu Li,Jian-Hua Wang,Yan-Song Pu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GRLF1 antibody

Immunoprecipitation - Anti-GRLF1 antibody (AB85950)

Western blot - Anti-GRLF1 antibody (AB85950)

Western blot - Anti-GRLF1 antibody (AB85950)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

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Primary Antibodies

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