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AB325866

Anti-GRM5 antibody [HL2543] - BSA and Azide free

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Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human GRM5.

View Alternative Names

GPRC1E, MGLUR5, GRM5, Metabotropic glutamate receptor 5, mGluR5

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded human breast carcinoma. mGluR5 stained by ab325866 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat hippocampus. mGluR5 stained by ab325866 diluted at 1 : 200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat striatum. mGluR5 stained by ab325866 diluted at 1 : 200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. mGluR5 stained by ab325866 diluted at 1 : 300. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. mGluR5 stained by ab325866 diluted at 1 : 300. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat brain. mGluR5 stained by ab325866 diluted at 1 : 200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. mGluR5 stained by ab325866 diluted at 1 : 300. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

ab325866 detects mGluR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. mGluR5 stained by ab325866 diluted at 1 : 300. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325866 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GRM5 antibody [HL2543] - BSA and Azide free (ab325866) at 1/500 dilution

Lane 1:

Unboiled mouse brain whole cell extracts at 50 µg

Lane 2:

Unboiled mouse liver whole cell extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)
  • WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2543] - BSA and Azide free (AB325866)

Boiled and unboiled various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325866 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GRM5 antibody [HL2543] - BSA and Azide free (ab325866) at 1/1000 dilution

Lane 1:

Boiled mouse brain tissue extracts at 50 µg

Lane 2:

Unboiled mouse brain various tissue extracts at 50 µg

Lane 3:

Boiled mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2543

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GRM5.

P41594

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
See full target information GRM5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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