Anti-GRM5 antibody [HL2876] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

ab325867 detects mGluR5 protein by immunofluorescent analysis. Sample : DIV11 rat E18 hippocampal neurons and glial cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : mGluR5 stained by ab325867 diluted at 1 : 250. Red : Tau, an axon marker, stained by Tau antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

Boiled and unboiled human tissue extract (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325867 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (ab325867) at 1/1000 dilution

Lane 1:

Boiled human brain tissue extract at 50 µg

Lane 2:

Unboiled human brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

Boiled and unboiled various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325867 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (ab325867) at 1/1000 dilution

Lane 1:

Boiled rat brain tissue extract at 50 µg

Lane 2:

Boiled rat liver tissue extract at 50 µg

Lane 3:

Unboiled rat brain tissue extract at 50 µg

Lane 4:

Unboiled rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325867 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (ab325867) at 1/1000 dilution

Lane 1:

Boiled mouse brain tissue extract at 50 µg

Lane 2:

Boiled mouse liver tissue extract at 50 µg

Lane 3:

Unboiled mouse brain tissue extract at 50 µg

Lane 4:

Unboiled mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ab325867 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (ab325867) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true