AB124898

Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CXCL1/GRO alpha antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

View Alternative Names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

1 Images

Supplier Data

Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (AB124898)

Exposure time : 3 minutes

Blocking buffer and concentartion : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST

Human GRO beta fragment protein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.

All lanes:

Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution

Lane 1:

Human GRO alpha active protein at 10 µg

Lane 2:

Human GRO beta fragment recombinant protein at 10 µg

Lane 3:

Human GRO gamma fragment recombinant protein at 10 µg

Secondary

Lanes 1 and 3:

Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution

Lane 2:

Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 10 kDa,36 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4902

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CXCL1 protein also known as GRO alpha GRO gamma and GRO beta functions as a chemokine involved in the recruitment and activation of neutrophils. The different variants share structural similarities though they have distinct biological effects. It weighs approximately 11 kDa. This target expresses in various tissues with high presence in epithelial cells and endothelial cells as well as in cells of the immune system. Its expression can be highly regulated in response to inflammatory stimuli and it can increase during immune responses.

Biological function summary

This target contributes to inflammation by attracting neutrophils to sites of tissue injury and infection aiding in the immune defense. It does not work as part of a larger complex but it can interact directly with G-protein coupled receptors on leukocytes. These interactions result in the activation of signaling pathways that lead to cell migration and proliferation supporting wound healing and tissue repair processes.

Pathways

CXCL1 is an important player in the chemokine signaling pathway where it interacts with the CXCR2 receptor triggering intracellular signaling cascades. It plays an instrumental role in the NF-kB pathway which regulates the expression of various downstream inflammatory mediators. This chemokine aligns functionally with other CXC chemokines like IL-8 sharing receptor binding to induce similar downstream effects that perpetuate inflammatory responses.

CXCL1 implicates in conditions such as chronic inflammation and cancer. Its overexpression can contribute to pathologies like rheumatoid arthritis where it perpetuates joint inflammation and helps sustain the inflammatory environment. Additionally elevated levels of CXCL1 may also exacerbate tumor progression and metastasis in certain cancers by promoting angiogenesis. In these disease contexts CXCL1 interacts with other inflammatory cytokines like TNF-alpha and IL-1 which together magnify the inflammatory or oncogenic signals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

See full target information CXCL1

Additional targets

CXCL3,CXCL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:16450 PubMed29180664

2017

Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Pasquier,Binitha Thomas,Jessica Hoarau-Véchot,Tala Odeh,Amal Robay,Omar Chidiac,Soha R Dargham,Rebal Turjoman,Anna Halama,Khalid Fakhro,Robert Menzies,Amin Jayyousi,Mahmoud Zirie,Jassim Al Suwaidi,Arash Rafii,Rayaz A Malik,Talal Talal,Charbel Abi Khalil

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]

Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (AB124898)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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Product promise