Anti-GRO gamma antibody [EPR18170-142]

• IP

Supplier Data

Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] (AB189486)

GRO gamma was immunoprecipitated from 0.35 mg mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant with ab189486 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 10 μl (Input).
Lane 2 : ab189486 IP in mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab189486 in Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] (ab189486)

Predicted band size: 11 kDa

false

• WB

Supplier Data

Western blot - Anti-GRO gamma antibody [EPR18170-142] (AB189486)

Blocking and dilution buffer/ concentration : 5% NFDM/TBST.

The culture supernatant was collected from a large culture dish and it was not concentrated.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution

Lane 1:

Untreated rat lung culture supernatant at 15 µL

Lane 2:

Rat lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6 days, culture supernatant at 15 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

true

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-GRO gamma antibody [EPR18170-142] (AB189486)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

The culture supernatant was collected from a large culture dish and it was not concentrated.

All lanes:

Western blot - Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution

Lane 1:

Untreated mouse lung culture supernatant at 15 µL

Lane 2:

Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant at 15 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

true

Exposure time: 3min