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AB242718

Anti-GRO gamma antibody [EPR18170-154] - BSA and Azide free

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Rabbit Recombinant Monoclonal GRO gamma antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Dcip1, Gm1960, Cxcl3, C-X-C motif chemokine 3, Dendritic cell inflammatory protein 1

2 Images
Sandwich ELISA - Anti-GRO gamma antibody [EPR18170-154] - BSA and Azide free (AB242718)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-GRO gamma antibody [EPR18170-154] - BSA and Azide free (AB242718)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab206310).

Sandwich ELISA - Anti-GRO gamma antibody [EPR18170-154] - BSA and Azide free (AB242718)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-GRO gamma antibody [EPR18170-154] - BSA and Azide free (AB242718)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272191).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18170-154

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab242718 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse GRO gamma (CXCL3) . The recommended pair for sandwich ELISA is:
Capture: ab242718, Mouse GRO gamma (CXCL3) Capture Antibody (unconjugated)
Detector: ab259524, Mouse GRO gamma (CXCL3) Detector Antibody (unconjugated)
The reference range value is 6.25 - 400 pg/ml.

Or alternatively, you can use:
Capture: ab242467, Mouse GRO gamma Capture Antibody (unconjugated)
Detector: ab242718, Mouse GRO gamma Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRO gamma also known as CXCL3 is a chemokine with a molecular weight of approximately 7.9 kDa. It is a small protein belonging to the CXC chemokine family. GRO gamma is expressed in numerous tissues including epithelial cells fibroblasts and several leukocytes. This protein plays a significant role in chemotactic signaling drawing neutrophils to sites of inflammation and infection acting as both a signal and effector molecule in immune response. Researchers often use CXCL3 ELISA kits and GRO protein assays to quantify its expression levels in biological samples.
Biological function summary

GRO gamma functions as a chemokine that guides neutrophils by binding to its receptor CXCR2. It participates in inflammatory processes and is produced in response to stimuli such as bacterial products and pro-inflammatory cytokines. GRO gamma together with its close family members forms a network facilitating the immune cell recruitment and activation at infection sites. Also this chemokine is included in larger chemokine complexes influencing not just immune cells but also modulating angiogenesis and cellular matrix restructuring.

Pathways

GRO gamma significantly contributes to the chemokine signaling pathway and the NF-kB signaling pathway. In these pathways it interacts tightly with proteins like CXCR2 which is essential for the signaling cascade that results in immune cell migration. It also engages with the MAPK pathways impacting cell proliferation and apoptosis. These involvements make GRO gamma vital for orchestrating the inflammation response in humans.

GRO gamma is linked to chronic inflammatory conditions and cancer. Elevated levels of GRO gamma have been observed in rheumatoid arthritis where its interaction with CXCR2 exacerbates inflammation and tissue damage. In cancer particularly in colorectal cancer GRO gamma plays a role in tumor progression by promoting angiogenesis and cell proliferation interacting with VEGF and other cytokines. Understanding its functions and relationships opens potential avenues for therapeutic interventions targeting chronic diseases and cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.
See full target information Cxcl3
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Product promise

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For full details, please see our Terms & Conditions

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