Anti-GRO gamma antibody [EPR29736-526]

• WB

Lab

Western blot - Anti-GRO gamma antibody [EPR29736-526] (AB325687)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of CXCL3 is upregulated in response to LPS treatment (PMID : 35789398).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-2 : 180 senconds, Lanes 3-4 : 10 seconds

All lanes:

Western blot - Anti-GRO gamma antibody [EPR29736-526] (ab325687) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 60 µg

Lane 2:

THP-1 treated first with 100ng/ml PMA for 24h, then replaced with 100ng/ml LPS for 4h, 1ug/ml BFA was then added for additional 3h, whole cell lysate at 60 µg

Lane 3:

Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 60 µg

Lane 4:

HUVEC treated with 0.5ug/ml LPS for 4h, 300ng/ml BFA was then added for additional 20h, whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 11 kDa,36 kDa

false

• I-ELISA

Lab

Indirect ELISA - Anti-GRO gamma antibody [EPR29736-526] (AB325687)

Indirect ELISA analysis of ab325687 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human CXCL3 protein;Human CXCL2 protein;Human CXCL1 protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human CXCL2 and human CXCL1.