AB259523

Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal GCP2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

GCP2, SCYB6, CXCL6, C-X-C motif chemokine 6, Chemokine alpha 3, Granulocyte chemotactic protein 2, Small-inducible cytokine B6, CKA-3, GCP-2, GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta

1 Images

Sandwich ELISA - Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector) (AB259523)

sELISA

Supplier Data

Sandwich ELISA - Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector) (AB259523)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260055).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22310-120

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

ab259523 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human GCP-2. The recommended pair for sandwich ELISA is:
Capture: ab244632, Human GCP-2 (CXCL6) Capture Antibody (unconjugated)
Detector: ab259523, Human GCP-2 (CXCL6) Detector Antibody (unconjugated)
The reference range value is 25 - 1600 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRO gamma also known as GCP-2 or CXCL3 is a chemokine with a mass of approximately 8 kDa. It is involved in the chemotactic activity attracting neutrophils to sites of inflammation. The protein is found mainly in various tissues like the liver lungs and kidneys where it plays central roles in immune response. GRO gamma interacts with chemokine receptors such as CXCR1 and CXCR2 which are key in transmitting signals that mediate cellular responses.

Biological function summary

GRO gamma plays a significant role in the regulation of immune and inflammatory responses. It is not a part of any larger protein complexes operating independently to exert its effects. GRO gamma induces chemotaxis in immune cells and is involved in the activation and recruitment of neutrophils from the bloodstream to the site of tissue damage or infection. It promotes the expression of adhesion molecules which aid in leukocyte migration across the endothelium.

Pathways

GRO gamma integrates into the broader chemokine signaling pathway and the inflammatory response pathway. It significantly influences the chemokine-mediated signaling pathway involving other chemokines like IL-8 (CXCL8) and related receptors. GRO gamma initiates signaling cascades that result in the activation of various signaling molecules leading to inflammatory responses and apoptosis of infected cells.

GRO gamma has been linked to conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). In these disorders GRO gamma takes part in the excessive recruitment of neutrophils contributing to tissue damage and inflammation. The protein shows functional overlap with cytokines such as TNF-alpha and IL-6 emphasizing its importance in understanding inflammatory diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

See full target information CXCL6

Additional targets

CXCL3

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector) (AB259523)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise