AB7762

Anti-Growth Cone antibody [2G13]

Name: Anti-Growth Cone antibody [2G13] (ab7762) | Abcam
Brand: Abcam Limited
SKU: AB7762
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal Chicken Growth Cone antibody. Suitable for ICC/IF, IHC-P, IHC-Fr, WB and reacts with Chicken, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Chicken Growth Cone protein.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Cone antibody [2G13] (AB7762)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Cone antibody [2G13] (AB7762)

Immunohistochemical staining of growth cones of primary olfactory neurons within the olfactory bulb in the developing rat brain using ab7762.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G13

Isotype

IgM

Carrier free

Reacts with

Rat, Mouse, Chicken

Applications

IHC-Fr, WB, IHC-P, ICC/IF

applications

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Reactivity data

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Product details

This antibody is useful as a general reagent for the detection of axonal growth cones in tissue sections and potentially a key marker in neuronal development research.

This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility. This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The growth cone also known as the neuronal growth cone is a dynamic structure found at the tip of a growing axon or dendrite in neurons. It weighs approximately 1.2 million Daltons and is comprised of a complex network of proteins and membrane-bound components. Growth cones are primarily expressed in the developing nervous system where they play an important role in guiding axons and dendrites to their synaptic targets. Essential molecules include actin and tubulin which provide structural support and are involved in movement through polymerization and depolymerization.

Biological function summary

Growth cones are critical for neuronal development and network formation. They function as sensory-motile organelles that explore the extracellular environment responding to guidance cues such as soluble gradients or substrate-bound cues. They translate these signals into directed movement and steering decisions for axonal pathways. Growth cones operate not just as individual units but are part of larger cellular complexes coordinating with other cytoskeletal structures and signaling molecules such as calcium ions and cyclic nucleotides to modify extension and retraction dynamics.

Pathways

Growth cones engage in key mechanisms like the Wnt signaling and PI3K/AKT pathways. These pathways modulate cytoskeleton remodeling and intracellular calcium levels which in turn influence growth cone motility and directionality. Within these pathways growth cones interact with proteins such as β-catenin in Wnt signaling providing cross-talk regulation necessary for synapse formation and neuronal plasticity.

Growth cone malfunction is associated with conditions such as spinal cord injuries and neurodegenerative diseases like Alzheimer's. Aberrant signaling within growth cones often involving proteins like amyloid precursor protein (APP) leads to impaired axonal guidance and regeneration. Understanding growth cone behavior and its association with pathology offers insights into potential therapeutic strategies for promoting neural repair and mitigating neurodegenerative progress.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Chicken Growth Cone

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 16:e0248309 PubMed33705493

2021

Direct interaction of HIV gp120 with neuronal CXCR4 and CCR5 receptors induces cofilin-actin rod pathology via a cellular prion protein- and NOX-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa K Smith,Isaac W Babcock,Laurie S Minamide,Alisa E Shaw,James R Bamburg,Thomas B Kuhn

The Journal of neuroscience : the official journal 40:1943-1955 PubMed31974206

2020

Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hemant Kumar,Chang Su Lim,Hyemin Choi,Hari Prasad Joshi,Kyoung-Tae Kim,Yong Ho Kim,Chul-Kyu Park,Hwan Myung Kim,In-Bo Han

Scientific reports 5:16866 PubMed26581550

2015

Dendritic cells and macrophages neurally hard-wired in the lymph node.

Applications

Unspecified application

Species

Unspecified reactive species

Clemens Wülfing,Hauke S Günther

Journal of neurocytology 28:1035-44 PubMed11054903

2000

Monoclonal antibody 2G13, a new axonal growth cone marker.

Applications

Unspecified application

Species

Unspecified reactive species

O Stettler,M S Bush,M Kasper,B Schlosshauer,P R Gordon-Weeks

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Growth Cone antibody [2G13]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Cone antibody [2G13] (AB7762)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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