AB9821

Anti-Growth Hormone antibody [GH-1]

Name: Anti-Growth Hormone antibody [GH-1] (ab9821) | Abcam
Brand: Abcam Limited
SKU: AB9821
Price: 525 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal Growth Hormone antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GH1.

View Alternative Names

Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1

2 Images

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Western blot - Anti-Growth Hormone antibody [GH-1] (AB9821)

Western blot of human growth hormone using ab9821 at a concentration of 1 μg/ml.

All lanes:

Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821)

Predicted band size: 25 kDa

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Western blot - Anti-Growth Hormone antibody [GH-1] (AB9821)

All lanes:

Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution

Lane 1:

Western blot - Recombinant human Growth Hormone protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-growth-hormone-protein-active-ab51232'>ab51232</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant human Growth Hormone protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-growth-hormone-protein-active-ab51232'>ab51232</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 25 kDa

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Exposure time: 2min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GH-1

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GH1.

P01241

Epitope

Ab9821 recognizes a different epitope than GH-2 (ab9822).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.

Biological function summary

GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.

Pathways

GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.

Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

See full target information GH1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Analytical and bioanalytical chemistry 414:6187-6200 PubMed35838770

2022

Selective quantification of the 22-kDa isoform of human growth hormone 1 in serum and plasma by immunocapture and LC-MS/MS.

Applications

Unspecified application

Species

Unspecified reactive species

Bas Sleumer,Martijn van Faassen,Michel J Vos,Rainer Bischoff,Ido P Kema,Nico C van de Merbel

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1144:122079 PubMed32247186

2020

Intact protein quantification in biological samples by liquid chromatography - high-resolution mass spectrometry: somatropin in rat plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Bults,Anders Sonesson,Magnus Knutsson,Rainer Bischoff,Nico C van de Merbel

International journal of molecular medicine 42:3037-3046 PubMed30272367

2018

Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Yanling Liu,Guolin Li,Zhengrong Chen,Guixiong Gu

Nature communications 7:10339 PubMed26842342

2016

A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Han Sun,Liqun Luo,Bachchu Lal,Xinrong Ma,Lieping Chen,Christine L Hann,Amy M Fulton,Daniel J Leahy,John Laterra,Min Li

Biosensors & bioelectronics 25:395-9 PubMed19692224

2009

Immobilization of specific monoclonal antibody on Au nanoparticles for hGH detection by electrochemical impedance spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Behzad Rezaei,Taghi Khayamian,Najmeh Majidi,Hamidreza Rahmani

Applied microbiology and biotechnology 84:693-9 PubMed19396439

2009

CHO-recombinant human growth hormone as a protease sensitive reporter protein.

Applications

Unspecified application

Species

Unspecified reactive species

Renate Kunert,Willibald Steinfellner,Friedrich Altmann,Jakob Wallner,Hermann Katinger,Karola Vorauer-Uhl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Growth Hormone antibody [GH-1]

Western blot - Anti-Growth Hormone antibody [GH-1] (AB9821)

Western blot - Anti-Growth Hormone antibody [GH-1] (AB9821)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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