AB202964

Anti-Growth hormone receptor antibody

Name: Anti-Growth hormone receptor antibody (ab202964) | Abcam
Brand: Abcam Limited
SKU: AB202964
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Growth hormone receptor antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GHR aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Growth hormone receptor, GH receptor, Somatotropin receptor, GHR

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth hormone receptor antibody (AB202964)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth hormone receptor antibody (AB202964)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat liver tissue labeling Growth hormone receptor with ab202964 at 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Growth hormone receptor antibody (AB202964)

Lysates probed with GHR Polyclonal Antibody, Unconjugated (ab202964) at 1/1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1 : 20000 for 60 min at 37˚C.

All lanes:

Western blot - Anti-Growth hormone receptor antibody (ab202964) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate

Lane 2:

Mouse cerebrum tissue lysate

Lane 3:

Rat testis tissue lysate

Lane 4:

Rat cerebrum tissue lysate

Predicted band size: 71.5 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, Pig, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GHR aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10912

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Use at 1/50 - 1/200 with fluorescent detection methods. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Growth Hormone Receptor (GHR) also known as somatotropin receptor is a vital protein that mediates the effects of growth hormone (GH). GHR is a transmembrane receptor with a mass of approximately 70 kDa and is expressed in many tissues including liver muscle and adipose tissue. It plays an important role in growth regulation by binding to growth hormone which triggers signal transduction pathways involved in cellular proliferation and growth.

Biological function summary

The growth hormone receptor facilitates the hormone's ability to promote growth and cell differentiation. The receptor when activated stimulates the JAK2/STAT5 signaling pathway a part of a larger GHR complex. This complex leads to gene transcription that stimulates growth and metabolism. The activation of GHR has significant effects on the cellular functions related to protein synthesis lipid metabolism and glucose metabolism.

Pathways

The growth hormone receptor is an important component of the JAK-STAT signaling pathway and the mTOR signaling pathway. In these pathways GHR interacts with other proteins such as Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5). These interactions play a critical role in translating the binding of growth hormone into cellular responses that lead to growth and metabolic regulation.

Disruptions or mutations in the growth hormone receptor can lead to conditions such as Laron syndrome and growth hormone insensitivity. These conditions often display symptoms of dwarfism and metabolic aberrations due to impaired signaling. GHR malfunction relates closely to growth hormone (GH) and anti-growth hormone treatments like somatropin used for managing growth deficiencies. The connection of GHR with related pathways and proteins like the IGF-1 (Insulin-like Growth Factor 1) underlines its role in metabolic diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth (PubMed : 1549776, PubMed : 2825030, PubMed : 8943276). On ligand binding, couples to the JAK2/STAT5 pathway (PubMed : 1549776, PubMed : 15690087, PubMed : 2825030, PubMed : 8943276).. Growth hormone-binding protein. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.. Isoform 2. Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling.

See full target information GHR

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

European journal of nutrition 59:2497-2506 PubMed31570976

2019

Maternal high-protein diet modulates hepatic growth axis in weaning piglets by reprogramming the IGFBP-3 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Rihua Cong,Xiaoli Qu,Hui Zhang,Yongling Hu,Silin Ye,Demin Cai,Xian Li,Hao-Yu Liu

Cell metabolism 28:310-323.e6 PubMed30043755

2018

Time-Dependent Molecular Responses Differ between Gastric Bypass and Dieting but Are Conserved Across Species.

Applications

Unspecified application

Species

Unspecified reactive species

Danny Ben-Zvi,Luca Meoli,Wasif M Abidi,Eirini Nestoridi,Courtney Panciotti,Erick Castillo,Palmenia Pizarro,Eleanor Shirley,William F Gourash,Christopher C Thompson,Rodrigo Munoz,Clary B Clish,Ron C Anafi,Anita P Courcoulas,Nicholas Stylopoulos

Journal of translational medicine 16:41 PubMed29482569

2018

Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Honghao Yu,Weihu Long,Xuezeng Zhang,Kaixiang Xu,Jianxiong Guo,Heng Zhao,Honghui Li,Yubo Qing,Weirong Pan,Baoyu Jia,Hong-Ye Zhao,Xingxu Huang,Hong-Jiang Wei

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Growth hormone receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth hormone receptor antibody (AB202964)

Western blot - Anti-Growth hormone receptor antibody (AB202964)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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