AB171089

Anti-Grp75/MOT antibody [9F8]

Name: Anti-Grp75/MOT antibody [9F8] (ab171089) | Abcam
Brand: Abcam Limited
SKU: AB171089

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(6 Publications)

Mouse Monoclonal Grp75/MOT antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Rat, African green monkey samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPA9.

View Alternative Names

GRP75, HSPA9B, mt-HSP70, HSPA9, 75 kDa glucose-regulated protein, Heat shock 70 kDa protein 9, Heat shock protein family A member 9, Mortalin, Peptide-binding protein 74, GRP-75, MOT, PBP74

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunofluorescence analysis of Grp75/MOT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab171089 at 1 : 50 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1 : 2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunofluorescent analysis of HeLa cells labeling Grp75/MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.

Supplier Data

Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (AB171089)

Detection of Grp75/MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2μg per 750μg lysate. Detected with ab171089 at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

All lanes:

Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1:

Untransfected HeLa cells

Lane 2:

HeLa cells transfected with scrambled siRNA

Lane 3:

HeLa cells transfected with Grp75/MOT siRNA

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

All lanes:

Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 30 µg

Lane 3:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 4:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

Lane 5:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 7:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 30 µg

Lane 8:

L6 (Rat skeletal muscle cell line) whole cell lysate at 30 µg

Lane 9:

Rat brain cell lysate at 30 µg

Lane 10:

Rat skeletal muscle cell lysate at 30 µg

Lane 11:

Rat liver cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

4-20% Tris-HCL polyacrylamide gel

All lanes:

Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 50 µg

Lane 4:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 50 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 50 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Lane 7:

NRK (Rat kidney cell line) whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Mouse HRP at 1/20000 dilution

Predicted band size: 74 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9F8

Isotype

IgG

Carrier free

Reacts with

Rat, Human, African green monkey

Applications

WB, IP, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HSPA9.

P38646

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 2μg.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Grp75 also known as MOT is a chaperone protein with a molecular mass of approximately 75 kDa. This protein is part of the heat shock protein 70 family and often referred to as Grp75 protein or MOT protein. Typically Grp75 is expressed in the mitochondria of eukaryotic cells. Its primary function lies in aiding the folding and assembly of newly synthesized proteins and responding to cellular stress conditions. The MOT-238 and MOT proteins are related variants in this protein family and antibodies like anti-MOT can specifically detect Grp75.

Biological function summary

Grp75 facilitates important cellular processes through its involvement in the regulation of mitochondrial homeostasis and cellular stress responses. Grp75 forms complexes with other mitochondrial proteins ensuring proper function of protein import and maintenance of mitochondrial DNA. It plays an essential role in the cellular response to stress by interacting with other heat shock proteins such as Hsp60 and by stabilizing proteins under conditions of thermal and oxidative stress. Additionally Grp75 is connected to the regulation of cell proliferation and apoptosis mechanisms integral to maintaining cellular balance.

Pathways

Grp75 is heavily involved in the mitochondrial unfolded protein response and apoptosis pathways. It associates with proteins like PINK1 and Parkin which are vital in the selective autophagy of mitochondria known as mitophagy. In response to misfolded proteins Grp75 helps to maintain mitochondrial function and promotes cell survival through interaction with other mitochondrial proteins. The JG1 and 9F8 proteins are also part of these pathways linked through their roles in protein quality control mechanisms within the cell.

Grp75 has been implicated in neurodegenerative diseases such as Parkinson's disease and certain cancers. Mutations or functional deficiencies in Grp75 can disrupt mitochondrial function which is a critical factor in the pathogenesis of Parkinson’s disease. The connection to proteins like PINK1 and Parkin in this context highlights the role Grp75 plays in the etiology of the disorder. In cancer Grp75 contributes to the cellular stress response and its overexpression can lead to drug resistance making it a target of interest for developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial chaperone that plays a key role in mitochondrial protein import, folding, and assembly. Plays an essential role in the protein quality control system, the correct folding of proteins, the re-folding of misfolded proteins, and the targeting of proteins for subsequent degradation. These processes are achieved through cycles of ATP binding, ATP hydrolysis, and ADP release, mediated by co-chaperones (PubMed : 18632665, PubMed : 25615450, PubMed : 28848044, PubMed : 30933555, PubMed : 31177526). In mitochondria, it associates with the TIM (translocase of the inner membrane) protein complex to assist in the import and folding of mitochondrial proteins (By similarity). Plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis, interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (PubMed : 26702583). Regulates erythropoiesis via stabilization of ISC assembly (PubMed : 21123823, PubMed : 26702583). Regulates mitochondrial calcium-dependent apoptosis by coupling two calcium channels, ITPR1 and VDAC1, at the mitochondria-associated endoplasmic reticulum (ER) membrane to facilitate calcium transport from the ER lumen to the mitochondria intermembrane space, providing calcium for the downstream calcium channel MCU, which releases it into the mitochondrial matrix (By similarity). Although primarily located in the mitochondria, it is also found in other cellular compartments. In the cytosol, it associates with proteins involved in signaling, apoptosis, or senescence. It may play a role in cell cycle regulation via its interaction with and promotion of degradation of TP53 (PubMed : 24625977, PubMed : 26634371). May play a role in the control of cell proliferation and cellular aging (By similarity). Protects against reactive oxygen species (ROS) (By similarity). Extracellular HSPA9 plays a cytoprotective role by preventing cell lysis following immune attack by the membrane attack complex by disrupting formation of the complex (PubMed : 16091382).

See full target information HSPA9

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:11062-11071 PubMed38975935

2024

Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

An Xie,Xianjie Zhang,Feng Ju,Yukai Zhou,Dan Wu,Jia Han

Frontiers in aging neuroscience 14:990679 PubMed36337694

2022

Sevoflurane exposure causes neuronal apoptosis and cognitive dysfunction by inducing ER stress activation of the inositol 1, 4, 5-trisphosphate receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Yanan Li,Xupeng Wang,Chunping Yin,Qi Zhou,Junfei Guo,Juan Zhao,Xiaohui Xian,Zhiyong Hou,Qiujun Wang

Cell death & disease 12:701 PubMed34262025

2021

ATF5, a putative therapeutic target for the mitochondrial DNA 3243A > G mutation-related disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xinpei Gao,Zhixin Jiang,Xinfeng Yan,Jiping Liu,Fengwen Li,Peng Liu,Jialu Li,Yuehua Wei,Yi Eve Sun,Yinan Zhang,Congrong Wang

Journal of veterinary science 19:528-535 PubMed29649860

2018

Identification of determinants that mediate binding between Tembusu virus and the cellular receptor heat shock protein A9.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmin Zhao,Qingtao Liu,Xinmei Huang,Huili Wang,Kaikai Han,Jing Yang,Keran Bi,Yuzhuo Liu,Lijiao Zhang,Yin Li

Molecular medicine reports 16:8595-8604 PubMed29039499

2017

An investigation of methyl tert‑butyl ether‑induced cytotoxicity and protein profile in Chinese hamster ovary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guangshan Xie,Wen-Xu Hong,Li Zhou,Xifei Yang,Haiyan Huang,Desheng Wu,Xinfeng Huang,Weiguo Zhu,Jianjun Liu

PloS one 11:e0150357 PubMed26959487

2016

Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects.

Applications

Species

Unspecified reactive species

Ainhoa Martínez-Pizarro,Lourdes R Desviat,Magdalena Ugarte,Belén Pérez,Eva Richard

View all publications

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Product promise

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Anti-Grp75/MOT antibody [9F8]

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (AB171089)

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise