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Mouse Monoclonal Grp75/MOT antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Rat, African green monkey samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPA9.

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Images

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089), expandable thumbnail
  • Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089), expandable thumbnail
  • Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089), expandable thumbnail
  • Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (AB171089), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human HSPA9. Database link P38646

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Rat
Expected
Tested
Expected
African green monkey
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Use 2μg.

Expected
Expected

Species
Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
African green monkey
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (PubMed:26702583). Regulates erythropoiesis via stabilization of ISC assembly (PubMed:21123823, PubMed:26702583). May play a role in cell cycle regulation via its interaction with and promotion of degradation of TP53 (PubMed:24625977, PubMed:26634371). May play a role in the control of cell proliferation and cellular aging (By similarity). Molecular adapter that regulates mitochondrial calcium-dependent apoptosis by coupling two calcium channels, ITPR1 and VDAC1, at the mitochondria-associated endoplasmic reticulum (ER) membrane to facilitate calcium transport from the ER lumen to the mitochondria intermembrane space, thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity).

Alternative names

GRP75, HSPA9B, mt-HSP70, HSPA9, 75 kDa glucose-regulated protein, Heat shock 70 kDa protein 9, Heat shock protein family A member 9, Mortalin, Peptide-binding protein 74, GRP-75, MOT, PBP74

Recommended products

Mouse Monoclonal Grp75/MOT antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Rat, African green monkey samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPA9.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human HSPA9. Database link P38646
Clone number
9F8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Grp75 also known as MOT is a chaperone protein with a molecular mass of approximately 75 kDa. This protein is part of the heat shock protein 70 family and often referred to as Grp75 protein or MOT protein. Typically Grp75 is expressed in the mitochondria of eukaryotic cells. Its primary function lies in aiding the folding and assembly of newly synthesized proteins and responding to cellular stress conditions. The MOT-238 and MOT proteins are related variants in this protein family and antibodies like anti-MOT can specifically detect Grp75.

Biological function summary

Grp75 facilitates important cellular processes through its involvement in the regulation of mitochondrial homeostasis and cellular stress responses. Grp75 forms complexes with other mitochondrial proteins ensuring proper function of protein import and maintenance of mitochondrial DNA. It plays an essential role in the cellular response to stress by interacting with other heat shock proteins such as Hsp60 and by stabilizing proteins under conditions of thermal and oxidative stress. Additionally Grp75 is connected to the regulation of cell proliferation and apoptosis mechanisms integral to maintaining cellular balance.

Pathways

Grp75 is heavily involved in the mitochondrial unfolded protein response and apoptosis pathways. It associates with proteins like PINK1 and Parkin which are vital in the selective autophagy of mitochondria known as mitophagy. In response to misfolded proteins Grp75 helps to maintain mitochondrial function and promotes cell survival through interaction with other mitochondrial proteins. The JG1 and 9F8 proteins are also part of these pathways linked through their roles in protein quality control mechanisms within the cell.

Associated diseases and disorders

Grp75 has been implicated in neurodegenerative diseases such as Parkinson's disease and certain cancers. Mutations or functional deficiencies in Grp75 can disrupt mitochondrial function which is a critical factor in the pathogenesis of Parkinson’s disease. The connection to proteins like PINK1 and Parkin in this context highlights the role Grp75 plays in the etiology of the disorder. In cancer Grp75 contributes to the cellular stress response and its overexpression can lead to drug resistance making it a target of interest for developing therapeutic strategies.

Product promise

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6 product images

  • Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089), expandable thumbnail

    Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)

    All lanes: Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1: Untransfected HeLa cells

    Lane 2: HeLa cells transfected with scrambled siRNA

    Lane 3: HeLa cells transfected with Grp75/MOT siRNA

    Secondary

    All lanes: Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Immunofluorescence analysis of Grp75/MOT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab171089 at 1:50 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

  • Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089), expandable thumbnail

    Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)

    All lanes: Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 30 µg

    Lane 3: A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Lane 4: K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

    Lane 5: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Lane 6: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Lane 7: COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 30 µg

    Lane 8: L6 (Rat skeletal muscle cell line) whole cell lysate at 30 µg

    Lane 9: Rat brain cell lysate at 30 µg

    Lane 10: Rat skeletal muscle cell lysate at 30 µg

    Lane 11: Rat liver cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

    Predicted band size: 74 kDa

  • Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089), expandable thumbnail

    Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)

    4-20% Tris-HCL polyacrylamide gel

    All lanes: Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 50 µg

    Lane 4: A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 50 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 50 µg

    Lane 6: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

    Lane 7: NRK (Rat kidney cell line) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat anti-Mouse HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 74 kDa

  • Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089), expandable thumbnail

    Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Detection of Grp75/MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2μg per 750μg lysate. Detected with ab171089 at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Immunofluorescent analysis of HeLa cells labeling Grp75/MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.

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